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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Mechanistic action of mesenchymal stem cell injection in the treatment of chemically induced ovarian failure in rabbits
Cytotherapy, Volume 15, No. 1, Year 2013
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Description
Background. No curative treatment is known for primary ovarian failure; however, mesenchymal stem cells (MSCs), through self-renewal and regeneration, push the trial to evaluate their role in the treatment of ovarian failure. The aim of this study was to explore the impact of MSCs on cyclophosphamide (CTX)-induced ovarian failure in rabbits and to clarify the mechanism(s) by which MSCs exert their action. Methods. Thirty-five adult female rabbits were injected with CTX to induce ovarian failure. Five rabbits were euthanized after the last injection of CTX for histological examination. The others (30 rabbits) were further subdivided into two groups: group 1 (ovarian failure group, 15 rabbits) received no treatment; group 2 (ovarian failure and MSC recipient group, 15 rabbits) received MSCs isolated from extracted bone marrow of male rabbits. Results. A decrease of follicle-stimulating hormone and an increase of estrogen and vascular endothelial growth factor (VEGF) levels in the MSC recipient group versus the ovarian failure group were found. Weak caspase-3 expression and +ve proliferating cell nuclear antigen staining after MSC injection were detected. Cytological and histological examinations showed increased follicle numbers with apparent normal structure of ovarian follicles in the MSC recipient group. Moreover, Y chromosomeecontaining cells from male donors were present within the ovarian tissues in group 2. Conclusions. The current study suggests that intravenous injection of MSCs into rabbits with chemotherapy-induced ovarian damage improved ovarian function. MSCs accomplish this function by direct differentiation into specific cellular phenotypes and by secretion of VEGF, which influence the regeneration of the ovary. © 2013, International Society for Cellular Therapy.
Authors & Co-Authors
Abd-Allah, Somia H.
Egypt, Zagazig
Zagazig University, Faculty of Medicine
Shalaby, Sally Mahmoud Saeed
Egypt, Zagazig
Zagazig University, Faculty of Medicine
Pasha, Heba Fouad
Egypt, Zagazig
Zagazig University, Faculty of Medicine
El-Shal, Amal S.
Egypt, Zagazig
Zagazig University, Faculty of Medicine
Raafat, Nermin Abd El Fattah
Egypt, Zagazig
Zagazig University, Faculty of Medicine
Shabrawy, Sheren M.
Egypt, Zagazig
Zagazig University, Faculty of Medicine
Awad, Hanan A.
Egypt, Zagazig
Zagazig University, Faculty of Medicine
Amer, Mona G.
Egypt, Giza
Faculty of Medicine
Gharib, Mahmoud A.
Egypt, Giza
Faculty of Medicine
El Gendy, Eman A.
Egypt, Giza
Faculty of Medicine
Raslan, Amal A.
Egypt, Banha
Faculty of Medicine
El-Kelawy, Hassan M.
Egypt, Zagazig
Zagazig University
Statistics
Citations: 84
Authors: 12
Affiliations: 4
Identifiers
Doi:
10.1016/j.jcyt.2012.08.001
ISSN:
14653249
e-ISSN:
14772566
Research Areas
Cancer
Participants Gender
Male
Female