Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Mechanisms controlling anaemia in Trypanosoma congolense infected mice
PLoS ONE, Volume 4, No. 4, Article e5170, Year 2009
Notification
URL copied to clipboard!
Description
Background: Trypanosoma congolense are extracellular protozoan parasites of the blood stream of artiodactyls and are one of the main constraints on cattle production in Africa. In cattle, anaemia is the key feature of disease and persists after parasitaemia has declined to low or undetectable levels, but treatment to clear the parasites usually resolves the anaemia. Methodology/Principal Findings: The progress of anaemia after Trypanosoma congolense infection was followed in three mouse strains. Anaemia developed rapidly in all three strains until the peak of the first wave of parasitaemia. This was followed by a second phase, characterized by slower progress to severe anaemia in C57BL/6, by slow recovery in surviving A/J and a rapid recovery in BALB/c. There was no association between parasitaemia and severity of anaemia. Furthermore, functional T lymphocytes are not required for the induction of anaemia, since suppression of T cell activity with Cyclosporin A had neither an effect on the course of infection nor on anaemia. Expression of genes involved in erythropoiesis and iron metabolism was followed in spleen, liver and kidney tissues in the three strains of mice using microarrays. There was no evidence for a response to erythropoietin, consistent with anaemia of chronic disease, which is erythropoietin insensitive. However, the expression of transcription factors and genes involved in erythropoiesis and haemolysis did correlate with the expression of the inflammatory cytokines Il6 and Ifng. Conclusions/Significance: The innate immune response appears to be the major contributor to the inflammation associated with anaemia since suppression of T cells with CsA had no observable effect. Several transcription factors regulating haematopoiesis, Tal1, Gata1, Zfpm1 and Klf1 were expressed at consistently lower levels in C57BL/6 mice suggesting that these mice have a lower haematopoietic capacity and therefore less ability to recover from haemolysis induced anaemia after infection. © 2009 Noyes et al.
Authors & Co-Authors
Noyes, Harry A.
United Kingdom, Liverpool
University of Liverpool
Alimohammadian, Mohammad H.
Iran, Tehran
Pasteur Institute of Iran
Agaba, Morris K.
Kenya, Nairobi
International Livestock Research Institute Nairobi
Brass, A.
United Kingdom, Manchester
The University of Manchester
Fuchs, Helmut
Germany, Oberschleissheim
Helmholtz Center Munich German Research Center for Environmental Health
Gailus-Durner, Valérie
Germany, Oberschleissheim
Helmholtz Center Munich German Research Center for Environmental Health
Hulme, Helen
United Kingdom, Manchester
The University of Manchester
Iraqi, Fuad A.
Kenya, Nairobi
International Livestock Research Institute Nairobi
Israel, Tel Aviv-yafo
Tel Aviv University
Kemp, Stephen J.
United Kingdom, Liverpool
University of Liverpool
Kenya, Nairobi
International Livestock Research Institute Nairobi
Rathkolb, Birgit
Germany, Oberschleissheim
Helmholtz Center Munich German Research Center for Environmental Health
Germany, Munich
Ludwig-maximilians-universität München
Wolf, Eckhard
Germany, Munich
Ludwig-maximilians-universität München
de Angelis, Martin Hrabé
Germany, Munich
Ludwig-maximilians-universität München
Germany, Munich
Technische Universität München
Roshandel, Delnaz
United Kingdom, Manchester
The University of Manchester
Naessens, Jan
Kenya, Nairobi
International Livestock Research Institute Nairobi
Statistics
Citations: 60
Authors: 14
Affiliations: 8
Identifiers
Doi:
10.1371/journal.pone.0005170
e-ISSN:
19326203