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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Rapid and Efficient Identification of Agrobacterium Species by recA Allele Analysis
Microbial Ecology, Volume 60, No. 4, Year 2010
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Description
The analysis of housekeeping recA gene sequences from 138 strains from 13 species or genomic species of Agrobacterium, nine being biovar 1 genomospecies, and the others Agrobacterium larrymoorei, Agrobacterium rubi, Agrobacterium sp. NCPPB 1650, and Agrobacterium vitis and one "former" Agrobacterium species, Rhizobium rhizogenes, led to the identification of 50 different recA alleles and to a clear delineation of the 14 species or genomospecies entirely consistent with that obtained by amplified fragment length polymorphism (AFLP) analysis. The relevance of a recA sequencing approach for epidemiological analyses was next assessed on agrobacterial Tunisian isolates. All Tunisian isolates were found to belong to the Agrobacterium tumefaciens/biovar 1 species complex by both biochemical tests and rrs sequencing. recA sequence analysis further permitted their unambiguous assignment to A. tumefaciens genomospecies G4, G6, G7, and G8 in total agreement with the results of an AFLP-based analysis. At subspecific level, several Tunisian recA alleles were novel, indicating the power and accuracy of recA-based typing for studies of Agrobacterium spp. © 2010 Springer Science+Business Media, LLC.
Authors & Co-Authors
Costechareyre, Denis
France, Villeurbanne
Université Claude Bernard Lyon 1
Rhouma, Ali
Tunisia, Tunis
Olive Tree Institute
Lavire, Céline
France, Villeurbanne
Université Claude Bernard Lyon 1
Portier, Perrine
France, Villeurbanne
Université Claude Bernard Lyon 1
France, Angers
Université D’angers
Chapulliot, David
France, Villeurbanne
Université Claude Bernard Lyon 1
Bertolla, Franck
France, Villeurbanne
Université Claude Bernard Lyon 1
Boubaker, A.
Tunisia, Tunis
University of Carthage, Institut National Agronomique de Tunisie
Dessaux, Yves
France, Paris
Cnrs Centre National de la Recherche Scientifique
Nesme, Xavier
France, Villeurbanne
Université Claude Bernard Lyon 1
Statistics
Citations: 85
Authors: 9
Affiliations: 5
Identifiers
Doi:
10.1007/s00248-010-9685-7
ISSN:
00953628
Research Areas
Genetics And Genomics