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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
International External Quality Assessment of Molecular Detection of Rift Valley Fever Virus
PLoS Neglected Tropical Diseases, Volume 7, No. 5, Article e2244, Year 2013
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Description
Rift Valley fever (RVF) is a viral zoonosis that primarily affects animals resulting in considerable economic losses due to death and abortions among infected livestock. RVF also affects humans with clinical symptoms ranging from an influenza-like illness to a hemorrhagic fever. Over the past years, RVF virus (RVFV) has caused severe outbreaks in livestock and humans throughout Africa and regions of the world previously regarded as free of the virus. This situation prompts the need to evaluate the diagnostic capacity and performance of laboratories worldwide. Diagnostic methods for RVFV detection include virus isolation, antigen and antibody detection methods, and nucleic acid amplification techniques. Molecular methods such as reverse-transcriptase polymerase chain reaction and other newly developed techniques allow for a rapid and accurate detection of RVFV. This study aims to assess the efficiency and accurateness of RVFV molecular diagnostic methods used by expert laboratories worldwide. Thirty expert laboratories from 16 countries received a panel of 14 samples which included RVFV preparations representing several genetic lineages, a specificity control and negative controls. In this study we present the results of the first international external quality assessment (EQA) for the molecular diagnosis of RVF. Optimal results were reported by 64% of the analyses, 21% of the analyses achieved acceptable results and 15% of the results revealed that there is need for improvement. Evenly good performances were achieved by specific protocols which can therefore be recommended as an accurate molecular protocol for the diagnosis of RVF. Other protocols showed uneven performances revealing the need for improved optimization and standardization of these protocols. © 2013 Escadafal et al.
Authors & Co-Authors
Escadafal, Camille
Germany, Berlin
Robert Koch Institute
Pawȩska, Janusz Tadeusz
South Africa, Johannesburg
National Institute for Communicable Diseases
South Africa, Johannesburg
School of Pathology
Grobbelaar, Antoinette A.
South Africa, Johannesburg
National Institute for Communicable Diseases
le Roux, C.
South Africa, Johannesburg
National Institute for Communicable Diseases
Bouloy, Michèle B.
France, Paris
Institut Pasteur, Paris
Patel, Pranav
Germany, Berlin
Robert Koch Institute
Teichmann, Anette
Germany, Berlin
Robert Koch Institute
Donoso-Mantke, Oliver
Germany, Berlin
Robert Koch Institute
Niedrig, Matthias
Germany, Berlin
Robert Koch Institute
Statistics
Citations: 27
Authors: 9
Affiliations: 4
Identifiers
Doi:
10.1371/journal.pntd.0002244
ISSN:
19352727
e-ISSN:
19352735
Research Areas
Genetics And Genomics
Infectious Diseases
Study Approach
Systematic review