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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Virus and host factors affecting the clinical outcome of bluetongue virus infection
Journal of Virology, Volume 88, No. 18, Year 2014
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Description
Bluetongue is a major infectious disease of ruminants caused by bluetongue virus (BTV), an arbovirus transmitted by Culicoides. Here, we assessed virus and host factors influencing the clinical outcome of BTV infection using a single experimental framework. We investigated how mammalian host species, breed, age, BTV serotypes, and strains within a serotype affect the clinical course of bluetongue. Results obtained indicate that in small ruminants, there is a marked difference in the susceptibility to clinical disease induced by BTV at the host species level but less so at the breed level. No major differences in virulence were found between divergent serotypes (BTV-8 and BTV-2). However, we observed striking differences in virulence between closely related strains of the same serotype collected toward the beginning and the end of the European BTV-8 outbreak. As observed previously, differences in disease severity were also observed when animals were infected with either blood from a BTV-infected animal or from the same virus isolated in cell culture. Interestingly, with the exception of two silent mutations, full viral genome sequencing showed identical consensus sequences of the virus before and after cell culture isolation. However, deep sequencing analysis revealed a marked decrease in the genetic diversity of the viral population after passaging in mammalian cells. In contrast, passaging in Culicoides cells increased the overall number of low-frequency variants compared to virus never passaged in cell culture. Thus, Culicoides might be a source of new viral variants, and viral population diversity can be another factor influencing BTV virulence. © 2014 Caporale et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC4178883/bin/supp_88_18_10399__index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC4178883/bin/JVI.01641-14_zjv999099491so1.pdf
Authors & Co-Authors
Caporale, Marco
Unknown Affiliation
Di Gialleonardo, Luigina
Unknown Affiliation
Janowicz, Anna
Unknown Affiliation
Wilkie, Gavin S.
Unknown Affiliation
Shaw, Andrew E.
Unknown Affiliation
Savini, Giovanni
Unknown Affiliation
Van Rijn, P. A.
Unknown Affiliation
Mertens, Peter Paul Clement
Unknown Affiliation
Di Ventura, M.
Unknown Affiliation
Palmarini, Massimo
Unknown Affiliation
Statistics
Citations: 91
Authors: 10
Affiliations: 6
Identifiers
Doi:
10.1128/JVI.01641-14
ISSN:
0022538X
e-ISSN:
10985514
Research Areas
Genetics And Genomics
Study Design
Cross Sectional Study