Physiological and chemical studies on mould proteins - II. Extraction and fractionation of the proteins of mould spores

A series of experiments was done to determine the best conditions for the extraction of protein from mould spores (Penicillium roqueforti). These experiments included studies on the influence of the extraction technique, the nature of the extracting fluid, spores glass: beads: extracting fluid ratio, and the time of extraction. Some work was done in fractionating the extractable nitrogenous substances and proteins of the spores. It was found that the extracted nitrogen could be fractionated into 12% dialysable low molecular weight nitrogen compounds and 88% crude protein, the latter was made up of about 40% albumens and 60% globulins. © 1962 Springer-Verlag.