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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
A real-time reverse transcription loop-mediated isothermal amplification assay for the rapid detection of yellow fever virus
Journal of Virological Methods, Volume 193, No. 1, Year 2013
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Description
Yellow fever, a mosquito-borne disease, is an important viral hemorrhagic fever in Africa and South America where it is endemic. Detection of yellow fever virus (YFV) in Africa remains a challenge due to a lack of highly specific tests. The aim of this study was to develop and optimize a rapid detection reverse transcription loop-mediated isothermal amplification (RT-LAMP) for YFV. The RT-LAMP was done isothermally at 62. °C using a real-time turbidimeter that allowed detection within 1. h. Specificity of the RT-LAMP was determined using RNA from flaviviruses and other related viruses where only YFV RNA was detected: West Nile virus, dengue viruses, Japanese encephalitis virus, Rift Valley fever virus, and chikungunya virus. In addition, equal sensitivity was also observed when the RT-LAMP and the real-time RT-PCR were compared using YFV-spiked human serum samples with a detection limit of 0.29 PFU/ml. Two Kenyan YFV wild strains showed an equal detection limit as the vaccine strain 17D in this study. The RT-LAMP reduced the time of reaction from 3. h to 1. h and increased sensitivity tenfold compared to RT-PCR. Therefore, this test offers a simple, rapid and reliable diagnostic tool for yellow fever when there are outbreaks of acute hemorrhagic fever in Kenya and other African countries. © 2013.
Authors & Co-Authors
Kwallah, Allan Ole
Kenya, Nairobi
Jomo Kenyatta University of Agriculture and Technology
Kenya, Nairobi
Kenya Medical Research Institute
Inoue, Shingo
Japan, Nagasaki
Nagasaki University
Muigai, Anne Wangari Thairu
Kenya, Nairobi
Jomo Kenyatta University of Agriculture and Technology
Kubo, T.
Japan, Nagasaki
Nagasaki University
Sang, Rosemary C.
Kenya, Nairobi
Kenya Medical Research Institute
Morita, Kouichi
Japan, Nagasaki
Nagasaki University
Mwau, Matilu M.
Kenya, Nairobi
Kenya Medical Research Institute
Statistics
Citations: 53
Authors: 7
Affiliations: 3
Identifiers
Doi:
10.1016/j.jviromet.2013.05.004
ISSN:
01660934
e-ISSN:
18790984
Research Areas
Infectious Diseases
Study Locations
Kenya