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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Evaluation of Aldrithiol-2-inactivated preparations of HIV type 1 subtypes A, B, and D as reagents to monitor T cell responses
AIDS Research and Human Retroviruses, Volume 23, No. 4, Year 2007
Notification
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Description
The development of HIV vaccines is an urgent priority and there is need to generate reagents representing multiple subtypes that can be used to screen HIV-1-specific responses. We used Aldrithiol-2 (AT-2), a mild oxidizing reagent, to eliminate the infectivity of HIV while maintaining its structure and ability to be processed for presentation to T cells. Inactivated subtype A, B, and D viruses were evaluated for their ability to stimulate T cell responses in PBMC samples from 18 U.S. subjects infected with HIV-1 subtype B and 32 Ugandan subjects infected with subtypes A and D or recombinants AC and AD. Five HIV-1-negative samples were also analyzed. T cell responses to AT-2-inactivated viral isolates were monitored by interferon-gamma (IFN-γ) intracellular cytokine secretion (ICS) analysis; matched microvesicle preparations served as negative controls. Among the 18 subtype B infected subjects, 39% had CD3 +CD4+ IFN-γ responses and 67% had CD3 +CD8+ IFN-γ responses. Of the 32 Ugandan subjects, 34% demonstrated CD3+CD4+ IFN-γ responses and 78% demonstrated CD3+CD8+ IFN-γ responses. Both subtype-specific and cross-reactive responses were observed. Responses to the AT-2 viruses tended to be lower in magnitude than those detected by a set of overlapping gag peptides. Robust lymphoproliferative responses to AT-2 viruses were seen in a subset of subjects. In conclusion, AT-2-inactivated HIV-1 virions stimulated both CD4 and CD8 HIV-1-specific responses and may provide an additional reagent for screening HIV-1-specific responses in HIV seropositives and vaccinees. © Mary Ann Liebert, Inc.
Authors & Co-Authors
Rutebemberwa, Alleluiah
United States, Rockville
Henry Jackson Foundation
Bess, J. W.
United States, Frederick
Saic-frederick
Brown, Bruce K.
United States, Rockville
Henry Jackson Foundation
Arroyo, Miguel A.
United States, Rockville
Henry Jackson Foundation
Eller, Michael A.
Uganda, Kampala
Makerere University
Slike, Bonnie M.
United States, Rockville
Henry Jackson Foundation
Polonis, Victoria R.
United States, Rockville
Henry Jackson Foundation
McCutchan, Francine E.
United States, Rockville
Henry Jackson Foundation
Currier, Jeffrey R.
United States, Rockville
Henry Jackson Foundation
Birx, Deborah L.
United States, Rockville
Henry Jackson Foundation
Robb, Merlin Lee
United States, Rockville
Henry Jackson Foundation
Marovich, Mary Anne
United States, Rockville
Henry Jackson Foundation
Lifson, Jeffrey D.
United States, Frederick
Saic-frederick
Cox, Josephine H.
United States, Rockville
Henry Jackson Foundation
Statistics
Citations: 21
Authors: 14
Affiliations: 3
Identifiers
Doi:
10.1089/aid.2006.0136
ISSN:
08892229
Research Areas
Infectious Diseases