Polyphenols content, antioxidant and antimicrobial activities of Ampelocissus grantii (Baker) Planch. (Vitaceae): A medicinal plant from Burkina Faso

The main goal of this study was to determine the phenol content, the antimicrobial and the antioxidant activities of acetone and water extracts from the rhizomes of Ampelocissus grantii (Baker) Planch (Vitaceae). The polyphenol content of extracts was determined colorimetrically using Folin-Ciocalteu method. The antioxidant activity of the same extracts was also screened using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method. The antimicrobial activity was performed by disc diffusion and broth microdilution assays against 9 reference bacterial strains including gram-negative and gram-positive bacteria and 1 fungal strain. The acetone extract exhibited the higher level of Total Polyphenol Contents (TPC) which correlated with strong antioxidant activity (IC50 = 10.87 ug mL-1 and AAI = 5.6) and antimicrobial activity. The polyphenol content of the acetone extract was 124.26 gallic acid equivalent/100 g (GAE/100 g), that of water extract was 74.25 GAE/100 g. The largest diameter of inhibition zone (DIZ) (15 mm) and the lowest Minimal Inhibitory Concentration (MIC) value (0.625 mg mL-1) were recorded withEnterococcusfaecalis 10907 CIP and Bacillus subtilus ATCC 21332. This study is a scientific base on the use of this plant in traditional health cure system. © 2010 Asian Network for Scientific Information.