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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Cadmium stress responses in Brassica juncea: Hints from proteomics and metabolomics
Journal of Proteome Research, Volume 12, No. 11, Year 2013
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Description
Among heavy metal stressors, cadmium (Cd) pollution is one leading threat to the environment. In this view, research efforts have been increasingly put forward to promote the individuation of phytoextractor plants that are capable of accumulating and withstanding the toxic metals, including Cd, in the aerial parts. We hereby adopted the hyperaccumulator B. juncea (Indian mustard) as a model to investigate plant responses to Cd stress at low (25 μM) and high (100 μM) doses. Analytical strategies included mass-spectrometry-based determination of Cd and the assessment of its effect on the leaf proteome and metabolome. Results were thus integrated with routine physiological data. Taken together, physiology results highlighted the deregulation of photosynthesis efficiency, ATP synthesis, reduced transpiration, and the impairment of light-independent carbon fixation reactions. These results were supported at the proteomics level by the observed Cd-dependent alteration of photosystem components and the alteration of metabolic enzymes, including ATP synthase subunits, carbonic anhydrase, and enzymes involved in antioxidant responses (especially glutathione and phytochelatin homeostasis) and the Calvin cycle. Metabolomics results confirmed the alterations of energy-generating metabolic pathways, sulfur-compound metabolism (GSH and PCs), and Calvin cycle. Besides, metabolomics results highlighted the up-regulation of phosphoglycolate, a byproduct of the photorespiration metabolism. This was suggestive of the likely increased photorespiration rate as a means to cope with Cd-induced unbalance in stomatal conductance and deregulation of CO2 homeostasis, which would, in turn, promote CO2 depletion and O2 (and thus oxidative stress) accumulation under prolonged photosynthesis in the leaves from plants exposed to high doses of CdCl2. Overall, it emerges that Cd-stressed B. juncea might rely on photorespiration, an adaptation that would prevent the over-reduction of the photosynthetic electron transport chain and photoinhibition. © 2013 American Chemical Society.
Authors & Co-Authors
D’Alessandro, Angelo
Italy, Viterbo
Università Degli Studi Della Tuscia Viterbo
Taamalli, Manel
Tunisia, Hamam Lif
Centre de Biotechnologie de Borj Cédria
Gevi, Federica
Italy, Viterbo
Università Degli Studi Della Tuscia Viterbo
Timperio, Anna Maria
Italy, Viterbo
Università Degli Studi Della Tuscia Viterbo
Zolla, Lello
Italy, Viterbo
Università Degli Studi Della Tuscia Viterbo
Ghnaya, Tahar
Tunisia, Hamam Lif
Centre de Biotechnologie de Borj Cédria
Statistics
Citations: 91
Authors: 6
Affiliations: 2
Identifiers
Doi:
10.1021/pr400793e
ISSN:
15353893
e-ISSN:
15353907
Research Areas
Environmental
Noncommunicable Diseases