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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Aspirin is an efficient inhibitor of quorum sensing, virulence and toxins in Pseudomonas aeruginosa
Microbial Pathogenesis, Volume 74, No. 1, Year 2014
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Description
Quorum sensing (QS) plays a vital role in regulation of virulence factors and toxins in Pseudomonas aeruginosa, which can cause serious human infections. Therefore, the QS system in P.aeruginosa may be an important target for pharmacological intervention. Activity of aspirin on the QS system was assessed using a reporter strain assay and confirmed using RT-PCR to test expression of virulence factors and toxins. In addition, molecular modeling techniques including docking, flexible alignment and surface mapping were also applied to further understand aspirin's potential QS inhibition activity. Aspirin (6mg/ml) showed significant reduction (p<0.01) of quorum sensing signals in P.aeruginosa, including expression of elastase, total proteases, and pyocyanin(p<0.01) without affecting bacterial viability. Aspirin also significantly reduced organism motility and biofilm production (p<0.01) and decreased expression of lasI, lasR, rhlI, rhlR, pqsA and pqsR genes by 38, 72, 69, 72, 74 and 43% respectively. Moreover, the expression of Pseudomonas toxins exoS and exoY was reduced by 47 and 55% respectively. The molecular modeling analysis suggests the QS inhibitory action of aspirin occurs through interaction of aspirin's aryl group and Tyr-88 of the LasR receptor, by strong π-π stacking interactions, which associated with a conformational change of the receptor-aspirin complex. The inhibitory effect of aspirin on virulence factors was specific to P.aeruginosa as aspirin at sub-MIC did not affect the biofilm or motility of Escherichia coli. To summarize, the collective data demonstrate that low concentrations of aspirin inhibit quorum sensing of P.aeruginosa. © 2014 Elsevier Ltd.
Authors & Co-Authors
El-Mowafy, Somaia A.
Egypt, Mansoura
Faculty of Pharmacy
Abd El Galil, Khaled Hussein
Egypt, Mansoura
Faculty of Pharmacy
El-Messery, Shahenda M.
Egypt, Mansoura
Faculty of Pharmacy
Egypt, Mansoura
Mansoura University
Shaaban, M. I.
Egypt, Mansoura
Faculty of Pharmacy
Statistics
Citations: 139
Authors: 4
Affiliations: 2
Identifiers
Doi:
10.1016/j.micpath.2014.07.008
e-ISSN:
10961208
Study Design
Randomised Control Trial