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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Mechanical loading-related changes in osteocyte sclerostin expression in mice are more closely associated with the subsequent osteogenic response than the peak strains engendered
Osteoporosis International, Volume 23, No. 4, Year 2012
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Description
Summary Osteocyte sclerostin is regulated by loading and disuse in mouse tibiae but ismore closely related to subsequent local osteogenesis than the peak strains engendered. Introduction The purpose of this study was to assess the relationship between loading-related change in osteocyte sclerostin expression, local strain magnitude, and local bone modeling/remodeling. Methods The right tibiae of 19-week-old female C57BL/6 mice were subjected to non-invasive, dynamic axial loading and/or to sciatic neurectomy-induced disuse. The sclerostin status of osteocytes was evaluated immunohistochemically, changes in bone mass by micro-computed tomography, new bone formation by histomorphometry, and loading-induced strain by strain gauges and finite element analysis. Results In cortical bone of the tibial shaft, loading engendered strains of similar peak magnitude proximally and distally. Proximally, sclerostin-positive osteocytes decreased and new bone formation increased. Distally, there was neither decrease in sclerostin-positive osteocytes nor increased osteogenesis. In trabecular bone of the proximal secondary spongiosa, loading decreased sclerostin-positive osteocytes and increased bone volume. Neither occurred in the primary spongiosa. Disuse increased sclerostin-positive osteocytes and decreased bone volume at all four sites. Loading reversed this sclerostin upregulation to a level below baseline in the proximal cortex and secondary spongiosa. Conclusion Loading-related sclerostin downregulation in osteocytes of the mouse tibia is associated preferentially with regions where new bone formation is stimulated rather than where high peak strains are engendered. The mechanisms involved remain unclear, but could relate to peak surface strains not accurately reflecting the strain-related osteogenic stimulus or that sclerostin regulation occurs after sufficient signal processing to distinguish between local osteogenic and non-osteogenic responses. © The Author(s) 2011.
Authors & Co-Authors
Moustafa, Alaa
United Kingdom, London
Royal Veterinary College University of London
Egypt, Kafr El-sheikh
Faculty of Veteinary Medicine
Sugiyama, T.
United Kingdom, London
Royal Veterinary College University of London
United Kingdom, Bristol
University of Bristol
Prasad, J.
United States, Seattle
University of Washington
Zaman, Gul
United Kingdom, London
Royal Veterinary College University of London
Gross, T. S.
United States, Seattle
University of Washington
Lanyon, Lance Edward
United Kingdom, London
Royal Veterinary College University of London
United Kingdom, Bristol
University of Bristol
Price, Joanna S.
United Kingdom, London
Royal Veterinary College University of London
United Kingdom, Bristol
University of Bristol
Statistics
Citations: 239
Authors: 7
Affiliations: 4
Identifiers
Doi:
10.1007/s00198-011-1656-4
ISSN:
0937941X
e-ISSN:
14332965
Participants Gender
Female