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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
general
High persister mutants in mycobacterium tuberculosis
PLoS ONE, Volume 11, No. 5, Article e0155127, Year 2016
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Description
Mycobacterium tuberculosis forms drug-tolerant persister cells that are the probable cause of its recalcitrance to antibiotic therapy. While genetically identical to the rest of the population, persisters are dormant, which protects them from killing by bactericidal antibiotics. The mechanism of persister formation in M. tuberculosis is not well understood. In this study, we selected for high persister (hip) mutants and characterized them by whole genome sequencing and transcriptome analysis. In parallel, we identified and characterized clinical isolates that naturally produce high levels of persisters. We compared the hip mutants obtained in vitro with clinical isolates to identify candidate persister genes. Genes involved in lipid biosynthesis, carbon metabolism, toxin-antitoxin systems, and transcriptional regulators were among those identified. We also found that clinical hip isolates exhibited greater ex vivo survival than the low persister isolates. Our data suggest that M. tuberculosis persister formation involves multiple pathways, and hip mutants may contribute to the recalcitrance of the infection.This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. © 2016 The work is made available under the Creative Commons CC0 public domain dedication.
Authors & Co-Authors
Via, L. E.
United States, Bethesda
National Institutes of Health Nih
Lee, Jongseok
South Korea, Seoul
International Tuberculosis Research Center
Lewis, Kim
United States, Boston
Northeastern University
Statistics
Citations: 117
Authors: 3
Affiliations: 3
Identifiers
Doi:
10.1371/journal.pone.0155127
ISSN:
19326203
Research Areas
Environmental
Health System And Policy
Study Design
Cross Sectional Study