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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Differences in proviral DNA load between HIV-1- and HIV-2-infected patients
AIDS, Volume 22, No. 2, Year 2008
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Description
INTRODUCTION: The lesser pathogenicity of HIV-2 relative to HIV-1 is generally attributed to its slower replication. To compare the amounts of total HIV DNA during human HIV-1 and HIV-2 infection, we developed a quantitative real-time PCR method with a unique external quantification standard based on a single plasmid harboring both the HIV-1 and the HIV-2 LTR. METHODS: Viral DNA load was compared between 40 HIV-1-infected and 42 HIV-2-infected antiretroviral-naive patients. RESULTS: The difference between HIV-1 and HIV-2 proviral DNA load was highly significant in patients with CD4 cell counts > 500 cells/μl [HIV-1: n = 14; median, 2.5; interquartile range (IQR), 2.1-2.7; HIV-2: n = 22, median, 1.6; IQR, 1.0-2.0] and in patients with CD4 cell counts between 300 cells/μl and 500 cells/μl (HIV-1: n = 12; median, 2.7; IQR, 2.3-2.8; HIV-2: n = 11; median, 2.0; IQR, 1.0-2.4). Too few HIV-2-infected patients had CD4 cell counts < 300 cells/μl to detect a significant difference but DNA values were again lower in HIV-2-infected patients (HIV-1: n = 14; median, 2.9; IQR, 2.2-3.2; HIV-2: n = 9; median, 2.7; IQR, 2.2-3.3). CONCLUSIONS: These differences are in line with the natural histories of the two infections and show that HIV-2 infection is a valid model for studying the pathophysiology of HIV infection in general. © 2008 Lippincott Williams & Wilkins, Inc.
Authors & Co-Authors
Gueudin, Marie
France, Rouen
Hopital Charles Nicolle
Damond, Florence
France, Paris
Hôpital Bichat-claude-bernard Ap-hp
Braun, Joséphine
France, Rouen
Hopital Charles Nicolle
Taïeb, Audrey
France, Paris
Inserm
Lémée, Véronique
France, Rouen
Hopital Charles Nicolle
Plantier, Jean Christophe
France, Rouen
Hopital Charles Nicolle
Chêne, Geneviève
France, Paris
Inserm
Matheron, Sophie
France, Paris
Hôpital Bichat-claude-bernard Ap-hp
Brun-Vézinet, Françoise
France, Paris
Hôpital Bichat-claude-bernard Ap-hp
Simon, Franćois
France, Rouen
Hopital Charles Nicolle
France, Paris
Hôpital Saint-louis
Statistics
Citations: 33
Authors: 10
Affiliations: 4
Identifiers
Doi:
10.1097/QAD.0b013e3282f42429
ISSN:
02699370
Research Areas
Genetics And Genomics
Infectious Diseases
Study Approach
Quantitative