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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Characterization and elimination of a TMV isolate infecting chrysanthemum plants in Egypt
International Journal of Virology, Volume 8, No. 1, Year 2012
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Description
An Egyptian isolate of a Tobamovirus was isolated and identified from Chrysanthemum cultivation in Egypt. Both biological, serological and sequence analysis of the coat protein gene demonstrated that the virus represents an isolate of the Tobamoviridae family. The isolated virus was nominated as TMV Chrysanthemum Egyptian isolate (TMV-Cfo-EG). This virus isolate caused severe disease symptoms in Chrysanthemum plants with mosaic, mottling and flower discoloration. The virus was purified biologically using serial transfer of the single local lesion technique on Nicotianagultinosa. The induced antiserum for the isolated virus had a titer 1X1024. 600 bp DNA fragments from the coat protein gene (CP) of TMV-Ch-EG was amplified with Rt-BCR technique. Bhylogenetic analysis of the TMV-Ch-EG/CB- gene showed 89% nucleotide sequence homology with other published strains of TMV in GenBank and 81% amino acid sequence homology. Tissue culture approach was used to permit the recovery of TMV-Ch-free micropropagated shoots via application of 20 mg L-1 virazole followed by thermotherapy at 38°C for two weeks and early screening to facilitate the efficient production of virus-free tissue culture derived propagules using the produced antiserum against TMV-Ch-EG. © 2012 Academic Journals Inc.
Authors & Co-Authors
Nassar, Entsar A.
Egypt, Helwan
Faculty of Science
El-Dougdoug, Khalid A.
Egypt, Cairo
College of Agriculture
Osman, Mohamed El Sayed
Egypt, Helwan
Faculty of Science
Dawoud, Rehab A.
Egypt, Giza
Agricultural Research Center
Kinawy, Aliaa H.
Egypt, Helwan
Faculty of Science
Statistics
Citations: 14
Authors: 5
Affiliations: 3
Identifiers
Doi:
10.3923/ijv.2012.14.26
Research Areas
Genetics And Genomics
Study Locations
Egypt