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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Cytogenetic and molecular aspects of absolute teratozoospermia: Comparison between polymorphic and monomorphic forms
Urology, Volume 78, No. 6, Year 2011
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Description
Objective: To compare the results of cytogenetic and molecular analysis between absolute polymorphic and monomorphic teratozoospermia. Methods: The semen samples from patients with polymorphic teratozoospermia (n = 20), globozoospermia (n = 8), or macrocephalic sperm head syndrome (n = 12), and healthy fertile men (n = 20) were analyzed according to the World Health Organization criteria. The constitutional blood karyotype of the patients was performed on cultured lymphocytes, according to standard techniques. Microdeletion analysis of the Y chromosomes used a sequence tagged site-polymerase chain reaction technique. Triple-color fluorescent in situ hybridization for chromosomes X, Y, and 18 were used to analyze the meiotic segregation. DNA fragmentation was detected using the terminal desoxynucleotidyl transferase-mediated deoxyuridine triphosphate biotin nick-end labeling assay. Results: Whatever the type of teratozoospermia, a normal karyotype and an absence of Y chromosome microdeletion were shown for all patients. A significant increase in the sperm aneuploidy rate and DNA fragmentation were shown, regardless of the type of teratozoospermia. Spermatozoa of the patients with globozoospermia carry an abnormal chromosomal constitution and DNA damage rate with the same frequency as that found in the sperm of patients with absolute polymorphic teratozoospermia. However, a greater sperm aneuploidy rate and DNA fragmentation were found in patients whose teratozoospermia was mainly characterized by increased rates of spermatozoa with macrocephalic head and multiple flagella. Conclusion: Our data have demonstrated that DNA fragmentation and sperm aneuploidy are critical tests in teratozoospermic men, because the results could negatively affect the intracytoplasmic sperm injection outcomes and might play an important role in the counseling of couples considering intracytoplasmic sperm injection. © 2011 Elsevier Inc.
Authors & Co-Authors
Brahem, Sonia
Tunisia, Sousse
Hopital Farhat Hached Sousse
Elghèzal, Hatem M.
Tunisia, Sousse
Hopital Farhat Hached Sousse
Ghédir, Houda
Tunisia, Sousse
Hopital Farhat Hached Sousse
Landolsi, Hanne
Tunisia, Sousse
Hopital Farhat Hached Sousse
Amara, Abdelbasset
Tunisia, Sousse
Hopital Farhat Hached Sousse
Ibala-Romdhane, S.
Tunisia, Sousse
Hopital Farhat Hached Sousse
Gribaa, Moez
Tunisia, Sousse
Hopital Farhat Hached Sousse
Saâd, Ali
Tunisia, Sousse
Hopital Farhat Hached Sousse
Mehdi, Meriem
Tunisia, Sousse
Hopital Farhat Hached Sousse
Statistics
Citations: 30
Authors: 9
Affiliations: 1
Identifiers
Doi:
10.1016/j.urology.2011.08.064
ISSN:
00904295
e-ISSN:
15279995
Research Areas
Genetics And Genomics
Participants Gender
Male