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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Avoidance of protein fold disruption in natural virus recombinants
PLoS Pathogens, Volume 3, No. 11, Year 2007
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Description
With the development of reliable recombination detection tools and an increasing number of available genome sequences, many studies have reported evidence of recombination in a wide range of virus genera. Recombination is apparently a major mechanism in virus evolution, allowing viruses to evolve more quickly by providing immediate direct access to many more areas of a sequence space than are accessible by mutation alone. Recombination has been widely described amongst the insect-transmitted plant viruses in the genus Begomovirus (family Geminiviridae), with potential recombination hot- and cold-spots also having been identified. Nevertheless, because very little is understood about either the biochemical predispositions of different genomic regions to recombine or what makes some recombinants more viable than others, the sources of the evolutionary and biochemical forces shaping distinctive recombination patterns observed in nature remain obscure. Here we present a detailed analysis of unique recombination events detectable in the DNA-A and DNA-A-like genome components of bipartite and monopartite begomoviruses. We demonstrate both that recombination breakpoint hot- and cold-spots are conserved between the two groups of viruses, and that patterns of sequence exchange amongst the genomes are obviously non-random. Using a computational technique designed to predict structural perturbations in chimaeric proteins, we demonstrate that observed recombination events tend to be less disruptive than sets of simulated ones. Purifying selection acting against natural recombinants expressing improperly folded chimaeric proteins is therefore a major determinant of natural recombination patterns in begomoviruses. © 2007 Lefeuvre et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC2092379/bin/ppat.0030181.sd001.zip
https://efashare.b-cdn.net/share/pmc/articles/PMC2092379/bin/ppat.0030181.sd002.zip
https://efashare.b-cdn.net/share/pmc/articles/PMC2092379/bin/ppat.0030181.sg001.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC2092379/bin/ppat.0030181.sg002.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC2092379/bin/ppat.0030181.st001.xls
https://efashare.b-cdn.net/share/pmc/articles/PMC2092379/bin/ppat.0030181.st002.xls
Authors & Co-Authors
Lefeuvre, Pierre F.
France, Saint-denis
Université de la Réunion
Lett, Jean Michel
France, Saint-denis
Université de la Réunion
Reynaud, Bernard
France, Saint-denis
Université de la Réunion
Martin, Darren Patrick
South Africa, Cape Town
University of Cape Town
Statistics
Citations: 134
Authors: 4
Affiliations: 2
Identifiers
Doi:
10.1371/journal.ppat.0030181
ISSN:
15537366
e-ISSN:
15537374
Research Areas
Cancer
Genetics And Genomics
Health System And Policy