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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
In vitro micropropagation and alkaloids of Hippeastrum vittatum
In Vitro Cellular and Developmental Biology - Plant, Volume 47, No. 6, Year 2011
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Description
Different concentrations of methyl jasmonate, spermine, casein hydrolysate, or progesterone combined with 16 mg/l 2 iP + 4 mg/l naphthalene acetic acid (NAA) were investigated in order to obtain higher multiplication rate and growth of Hippeastrum vittatum bulbs in vitro on MS basal medium. The highest multiplication rate (8.2 bulbs/explant) was attained with 80 mg/l spermine, while the highest bulb fresh weight (1.23 g/bulblet) was obtained with 4 mg/l methyl jasmonate. Progesterone at 20 mg/l or casein hydrolysate at 2.0 g/l gave the highest leaf length (14.1 and 13.2 cm, respectively). So, it can be advised to use 80 mg/l spermine combined with 16 mg/l 2 iP + 4 mg/l NAA to obtain the highest number of bulbs per explant with moderate leaf length and bulb fresh weight. Chemical analysis showed alternations in the alkaloid type ratio and number of compounds in the bulbs treated with methyl jasmonate (4 mg/l). © 2011 The Society for In Vitro Biology.
Authors & Co-Authors
Zayed, Rawia A.
Egypt, Zagazig
Faculty of Pharmacy
Egypt, El-arish
Faculty of Pharmacy
El-Shamy, Hesham A.
Egypt, Zagazig
Faculty of Agriculture
Berkov, Strahil Hristov
Spain, Barcelona
Universitat de Barcelona
Bulgaria, Sofia
Agrobioinstitute, Sofia
Bastida, J.
Spain, Barcelona
Universitat de Barcelona
Codina Jané, C.
Spain, Barcelona
Universitat de Barcelona
Statistics
Citations: 19
Authors: 5
Affiliations: 5
Identifiers
Doi:
10.1007/s11627-011-9368-1
ISSN:
10545476