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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Optimization, production, and partial characterization of an alkalophilic amylase produced by sponge associated marine bacterium Halobacterium salinarum MMD047
Biotechnology and Bioprocess Engineering, Volume 14, No. 1, Year 2009
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Description
An endosymbiont Halobacterium salinarum MMD047, which could produce high yields of amylase, was isolated from marine sponge Fasciospongia cavernosa, collected from the peninsular coast of India. Maximum production of enzyme was obtained in minimal medium supplemented with 1% sucrose. The enzyme was found to be produced constitutively even in the absence of starch. The optimum temperature and pH for the enzyme production was 40°C and 8.0, respectively. The enzyme exhibited maximum activity in pH range of 6∼10 with an optimum pH of 9.0. The enzyme was stable at 40°C and the enzyme activity decreased dramatically above 50°C. Based on the present findings, the enzyme was characterized as relatively heat sensitive and alkalophilic amylase which can be developed for extensive industrial applications. © The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg GmbH 2009.
Authors & Co-Authors
Shanmughapriya, Santhanam
India, Tiruchirappalli
Bharathidasan University
Seghal Kiran, G. Seghal
India, Tiruchirappalli
Bharathidasan University
Selvin, Joseph A.
India, Tiruchirappalli
Bharathidasan University
Manilal, Aseer
India, Tiruchirappalli
Bharathidasan University
Sujith, Sugathan
India, Tiruchirappalli
Bharathidasan University
Statistics
Citations: 38
Authors: 5
Affiliations: 1
Identifiers
Doi:
10.1007/s12257-008-0060-1
ISSN:
12268372