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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
pharmacology, toxicology and pharmaceutics
Influence of Sutherlandia frutescens extracts on cell numbers, morphology and gene expression in MCF-7 cells
Journal of Ethnopharmacology, Volume 112, No. 2, Year 2007
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Description
Sutherlandia frutescens is a well-known South African herbal remedy traditionally used for stomach problems, internal cancers, diabetes, various inflammatory conditions and recently to improve the overall health in cancer and HIV/AIDS patients. The influence of crude Sutherlandia frutescens extracts (prepared with 70% ethanol) was investigated on cell numbers, morphology, and gene expression profiles in a MCF-7 human breast adenocarcinoma cell line. Time-dependent (24, 34, 48 and 72 h) and dose-dependent (0.5-2.5 mg/ml) studies were conducted utilizing spectrophotometrical analysis with crystal violet as DNA stain. A statistically significant decrease to 50% of malignant cell numbers was observed after 24 h of exposure to 1.5 mg/ml Sutherlandia frutescens extract when compared to vehicle-treated controls. Morphological characteristics of apoptosis including cytoplasmic shrinking, membrane blebbing and apoptotic bodies were observed after 24 h of exposure. A preliminary global gene expression profile was obtained by means of microarray analysis and revealed valuable information about the molecular mechanisms and signal transduction associated with 70% ethanolic Sutherlandia frutescens extracts. © 2007 Elsevier Ireland Ltd. All rights reserved.
Authors & Co-Authors
Stander, André
South Africa, Pretoria
University of Pretoria
Marais, Sumari
South Africa, Pretoria
University of Pretoria
Steynberg, T. J.
South Africa, Pretoria
University of Pretoria
Theron, D.
South Africa, Pretoria
University of Pretoria
Joubert, Fourie
South Africa, Pretoria
University of Pretoria
Albrecht, Carl F.D.V.
South Africa, Johannesburg
Cancer Association of South Africa
Joubert, Anna Margaretha
South Africa, Pretoria
University of Pretoria
Statistics
Citations: 45
Authors: 7
Affiliations: 2
Identifiers
Doi:
10.1016/j.jep.2007.03.013
ISSN:
03788741
Research Areas
Cancer
Genetics And Genomics
Infectious Diseases
Noncommunicable Diseases