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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Purification and biochemical characterization of a new alkali-stable laccase from Trametes sp. isolated in Tunisia: Role of the enzyme in olive mill waste water treatment
World Journal of Microbiology and Biotechnology, Volume 29, No. 11, Year 2013
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Description
A white-rot basidiomycete, isolated from decayed acacia wood (from Northwest of Tunisia) and identified as Trametes sp, was selected in a broad plate screening because of its ability to decolorize and dephenolize olive oil mill wastewater (OMW) efficiently. The major laccase was purified and characterized as a monomeric protein with apparent molecular mass of 61 kDa (SDS-PAGE). It exhibits high enzyme activity over broad pH and temperature ranges with optimum activity at pH 4.0 and a temperature of 60 °C. The purified laccase is stable at alkaline pH values. The enzyme retained 50 % of its activity after 90 min of incubation at 55 °C. Using ABTS, this laccase presented K m and V max values of 0.05 mM and 212.73 μmoL min-1 mg-1, respectively. It has shown a degrading activity towards a variety of phenolic compounds. The purified laccase was partially inhibited by Fe2+, Zn2+, Cd2+ and Mn2+, while Cu2+ acted as inducer. EDTA (10 mM) and NaN3 (10 mM) were found to completely inhibit its activity. 73 % OMW was dephenolized after 315 min incubation at 30 °C with 2 U mL-1 of laccase and 2 mM HBT. © 2013 Springer Science+Business Media Dordrecht.
Authors & Co-Authors
Daâssi, Dalel
Tunisia, Sfax
Ecole Nationale D'ingénieurs de Sfax
Zouari-Mechichi, Héla
Tunisia, Sfax
Ecole Nationale D'ingénieurs de Sfax
Prieto, Alicia
Spain, Madrid
Csic - Centro de Investigaciones Biológicas Margarita Salas Cib
Martínez, María Jesús
Spain, Madrid
Csic - Centro de Investigaciones Biológicas Margarita Salas Cib
Nasri, Moncef
Tunisia, Sfax
Ecole Nationale D'ingénieurs de Sfax
Mechichi, Tahar
Tunisia, Sfax
Ecole Nationale D'ingénieurs de Sfax
Statistics
Citations: 37
Authors: 6
Affiliations: 2
Identifiers
Doi:
10.1007/s11274-013-1380-7
ISSN:
09593993
Research Areas
Environmental
Study Locations
Tunisia