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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Protein kinase C promotes restoration of calcium homeostasis to platelet activating factor-stimulated human neutrophils by inhibition of phospholipase C
Journal of Inflammation, Volume 6, Article 29, Year 2009
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Description
Background: The role of protein kinase C (PKC) in regulating the activity of phospholipase C (PLC) in neutrophils activated with the chemoattractant, platelet-activating factor (PAF, 20 and 200 nM), was probed in the current study using the selective PKC inhibitors, GF10903X (0.5 - 1 M) and staurosporine (400 nM). Methods: Alterations in cytosolic Ca2+, Ca2+ influx, inositol triphosphate (IP3), and leukotriene B4production were measured using spectrofluorimetric, radiometric and competitive binding radioreceptor and immunoassay procedures, respectively. Results: Activation of the cells with PAF was accompanied by an abrupt increase in cytosolic Ca 2+ followed by a gradual decline towards basal levels. Pretreatment of neutrophils with the PKC inhibitors significantly increased IP 3production with associated enhanced Ca2+ release from storage vesicles, prolongation of the peak cytosolic Ca2+ transients, delayed clearance and exaggerated reuptake of the cation, and markedly increased synthesis of LTB4. The alterations in Ca2+ fluxes observed with the PKC inhibitors were significantly attenuated by U73122, a PLC inhibitor, as well as by cyclic AMP-mediated upregulation of the Ca 2+-resequestering endomembrane ATPase. Taken together, these observations are compatible with a mechanism whereby PKC negatively modulates the activity of PLC, with consequent suppression of IP3production and down-regulation of Ca2+ mediated pro-inflammatory responses of PAF-activated neutrophils. Conclusion: Although generally considered to initiate and/or amplify intracellular signalling cascades which activate and sustain the pro-inflammatory activities of neutrophils and other cell types, the findings of the current study have identified a potentially important physiological, anti-inflammatory function for PKC, at least in neutrophils. © 2009 Tintinger et al; licensee BioMed Central Ltd.
Authors & Co-Authors
Tintinger, Gregory Ronald
South Africa, Pretoria
Faculty of Health Sciences
Theron, Annette Johanna
South Africa, Pretoria
University of Pretoria
Steel, Helen C.
South Africa, Pretoria
University of Pretoria
Cockeran, Riana
South Africa, Pretoria
University of Pretoria
Pretorius, L.
South Africa, Pretoria
University of Pretoria
Anderson, Ronald
South Africa, Pretoria
University of Pretoria
Statistics
Citations: 54
Authors: 6
Affiliations: 2
Identifiers
Doi:
10.1186/1476-9255-6-29
e-ISSN:
14769255