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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Ultrasensitive multi-analyte electrochemical immunoassay based on GNR-modified heated screen-printed carbon electrodes and PS@PDA-metal labels for rapid detection of MMP-9 and IL-6
Biosensors and Bioelectronics, Volume 55, Year 2014
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Description
An ultrasensitive electrochemical immunoassay was developed for rapid detection of interleukin-6 (IL-6) and matrix metallopeptidase-9 (MMP-9); the method utilized PS@PDA-metal nanocomposites based on graphene nanoribbon (GNR)-modified heated screen-printed carbon electrode (HSPCE). Because of the good hydrophilicity and low toxicity, GNRs were used to immobilize antibodies (Ab) and amplify the electrochemical signal. PS@PDA-metal was used to label antibodies and generate a strong electrochemical signal in acetic buffer. A sandwich strategy was adopted to achieve simultaneous detection of MMP-9 and IL-6 based on HSPCE without cross-talk between adjacent electrodes in the range of 10-5 to 103ngmL-1 with detection limits of 5fgmL-1 and 0.1pgmL-1 (S/N=3), respectively. The proposed method showed wide detection range, low detection limit, acceptable stability and good reproducibility. Satisfactory results were also obtained in the practical samples, thus showing this is a promising technique for simultaneous clinical detection of biocomponent proteins.© 2013 Elsevier B.V.
Authors & Co-Authors
Shi, Jian Jun
China, Huainan
Anhui University of Science and Technology
China, Nanjing
Nanjing University
He, Ting Ting
China, Huainan
Anhui University of Science and Technology
China, Nanjing
Nanjing University
Jiang, Fang
China, Nanjing
Nanjing University
Abdel-Halim, Essam Sayed
Saudi Arabia, Riyadh
College of Sciences
Zhu, Junjie
China, Nanjing
Nanjing University
Statistics
Citations: 65
Authors: 5
Affiliations: 3
Identifiers
Doi:
10.1016/j.bios.2013.11.056
ISSN:
09565663
e-ISSN:
18734235
Research Areas
Environmental