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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
A new antigen scanning strategy for monitoring HIV-1 specific T-cell immune responses
Journal of Immunological Methods, Volume 375, No. 1-2, Year 2012
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Description
Delineation of the immune correlates of protection in natural infection or after vaccination is a mandatory step for vaccine development. Although the most recent techniques allow a sensitive and specific detection of the cellular immune response, a consensus on the best strategy to assess their magnitude and breadth is yet to be reached. Within the AIDS Vaccine Integrated Project (AVIP http://www.avip-eu.org) we developed an antigen scanning strategy combining the empirical-based approach of overlapping peptides with a vast array of database information. This new system, termed Variable Overlapping Peptide Scanning Design (VOPSD), was used for preparing two peptide sets encompassing the candidate HIV-1 vaccine antigens Tat and Nef. Validation of the VOPSD strategy was obtained by direct comparison with 15mer or 20mer peptide sets in a trial involving six laboratories of the AVIP consortium. Cross-reactive background responses were measured in 80 HIV seronegative donors (HIV-), while sensitivity and magnitude of Tat and Nef-specific T-cell responses were assessed on 90 HIV+ individuals. In HIV-, VOPSD peptides generated background responses comparable with those of the standard sets. In HIV-1+ individuals the VOPSD pools showed a higher sensitivity in detecting individual responses (Tat VOPSD vs. Tat 15mers or 20mers: p ≤ 0.01) as well as in generating stronger responses (Nef VOPSD vs. Nef 20mers: p < 0.001) than standard sets, enhancing both CD4 and CD8 T-cell responses. Moreover, this peptide design allowed a marked reduction of the peptides number, representing a powerful tool for investigating novel HIV-1 candidate vaccine antigens in cohorts of HIV-seronegative and seropositive individuals. © 2011 Elsevier B.V.
Authors & Co-Authors
Malnati, Mauro S.
Italy, Milan
Irccs Ospedale San Raffaele
Heltai, Silvia
Italy, Milan
Irccs Ospedale San Raffaele
Cosma, Antonio
Germany, Oberschleissheim
Helmholtz Center Munich German Research Center for Environmental Health
Reitmeir, Peter
Germany, Oberschleissheim
Helmholtz Center Munich German Research Center for Environmental Health
Allgayer, Simone
Germany, Oberschleissheim
Helmholtz Center Munich German Research Center for Environmental Health
Germany, Munich
Technische Universität München
Glashoff, Richard Helmuth
South Africa, Stellenbosch
Stellenbosch University
Liebrich, Walter
South Africa, Stellenbosch
Stellenbosch University
Vardas, Eftyhia
South Africa, Johannesburg
University of the Witwatersrand Faculty of Health Sciences
Imami, Nesrina
United Kingdom, London
Imperial College London
Westrop, Samantha Jane
United Kingdom, London
Imperial College London
Nozza, Silvia
Italy, Milan
Irccs Ospedale San Raffaele
Tambussi, Giuseppe
Italy, Milan
Irccs Ospedale San Raffaele
Buttò, Stefano
Italy, Rome
Istituto Superiore Di Sanita
Fanales-Belasio, Emanuele
Italy, Rome
Istituto Superiore Di Sanita
Ensoli, Barbara
Italy, Rome
Istituto Superiore Di Sanita
Ensoli, Fabrizio
Italy, Rome
Irccs Istituto Dermatologico San Gallicano
Tripiciano, Antonella
Italy, Rome
Irccs Istituto Dermatologico San Gallicano
Fortis, Claudio
Italy, Milan
Irccs Ospedale San Raffaele
Lusso, Paolo
Italy, Milan
Irccs Ospedale San Raffaele
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Poli, Guido
Italy, Milan
Irccs Ospedale San Raffaele
Italy, Milan
Università Vita-salute San Raffaele
Erfle, Volker F.
Germany, Oberschleissheim
Helmholtz Center Munich German Research Center for Environmental Health
Holmes, Harvey C.
United Kingdom, London
Public Health England
Statistics
Citations: 22
Authors: 22
Affiliations: 11
Identifiers
Doi:
10.1016/j.jim.2011.09.005
ISSN:
00221759
e-ISSN:
18727905
Research Areas
Infectious Diseases
Study Design
Cohort Study