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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Host markers in Quantiferon supernatants differentiate active TB from latent TB infection: Preliminary report
BMC Pulmonary Medicine, Volume 9, Article 21, Year 2009
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Description
Background: Interferon gamma release assays, including the QuantiFERON® TB Gold In Tube (QFT) have been shown to be accurate in diagnosing Mycobacterium tuberculosis infection. These assays however, do not discriminate between latent TB infection (LTBI) and active TB disease. Methods: We recruited twenty-three pulmonary TB patients and 34 household contacts from Cape Town, South Africa and performed the QFT test. To investigate the ability of new host markers to differentiate between LTBI and active TB, levels of 29 biomarkers in QFT supernatants were evaluated using a Luminex multiplex cytokine assay. Results: Eight out of 29 biomarkers distinguished active TB from LTBI in a pilot study. Baseline levels of epidermal growth factor (EGF) soluble CD40 ligand (sCD40L), antigen stimulated levels of EGF, and the background corrected antigen stimulated levels of EGF and macrophage inflammatory protein (MIP)-1β were the most informative single markers for differentiation between TB disease and LTBI, with AUCs of 0.88, 0.84, 0.87, 0.90 and 0.79 respectively. The combination of EGF and MIP-1β predicted 96% of active TB cases and 92% of LTBIs. Combinations between EGF, sCD40L, VEGF, TGF-α and IL-1α also showed potential to differentiate between TB infection states. EGF, VEGF, TGF-α and sCD40L levels were higher in TB patients. Conclusion: These preliminary data suggest that active TB may be accurately differentiated from LTBI utilizing adaptations of the commercial QFT test that includes measurement of EGF, sCD40L, MIP-1β, VEGF, TGF-α or IL-1α in supernatants from QFT assays. This approach holds promise for development as a rapid diagnostic test for active TB. © 2009 Chegou et al; licensee BioMed Central Ltd.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC2696407/bin/1471-2466-9-21-S1.xls
https://efashare.b-cdn.net/share/pmc/articles/PMC2696407/bin/1471-2466-9-21-S2.xls
https://efashare.b-cdn.net/share/pmc/articles/PMC2696407/bin/1471-2466-9-21-S3.xls
https://efashare.b-cdn.net/share/pmc/articles/PMC2696407/bin/1471-2466-9-21-S4.xls
Authors & Co-Authors
Chegou, Novel N.
South Africa, Stellenbosch
Stellenbosch University
Black, Gillian Frances
South Africa, Stellenbosch
Stellenbosch University
Kidd, Martin
South Africa, Stellenbosch
Stellenbosch University
Van Helden, Paul D.
South Africa, Stellenbosch
Stellenbosch University
Walzl, Gerhard
South Africa, Stellenbosch
Stellenbosch University
Statistics
Citations: 178
Authors: 5
Affiliations: 1
Identifiers
Doi:
10.1186/1471-2466-9-21
e-ISSN:
14712466
Research Areas
Infectious Diseases
Study Locations
South Africa