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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Proximity of H2A.Z containing nucleosome to the transcription start site influences gene expression levels in the mammalian liver and brain
Nucleic Acids Research, Volume 40, No. 18, Year 2012
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Description
Nucleosome positioning maps of several organisms have shown that Transcription Start Sites (TSSs) are marked by nucleosome depleted regions flanked by strongly positioned nucleosomes. Using genomewide nucleosome maps and histone variant occupancy in the mouse liver, we show that the majority of genes were associated with a single prominent H2A.Z containing nucleosome in their promoter region. We classified genes into clusters depending on the proximity of H2A.Z to the TSS. The genes with no detectable H2A.Z showed lowest expression level, whereas H2A.Z was positioned closer to the TSS of genes with higher expression levels. We confirmed this relation between the proximity of H2A.Z and expression level in the brain. The proximity of histone variant H2A.Z, but not H3.3 to the TSS, over seven consecutive nucleosomes, was correlated with expression. Further, a nucleosome was positioned over the TSS of silenced genes while it was displaced to expose the TSS in highly expressed genes. Our results suggest that gene expression levels in vivo are determined by accessibility of the TSS and proximity of H2A.Z. © 2012 The Author(s).
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3467062/bin/supp_40_18_8965__index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC3467062/bin/supp_gks665_nar-00863-m-2012-File003.doc
Authors & Co-Authors
Bargaje, Rhishikesh
India, New Delhi
Institute of Genomics and Integrative Biology India
Alam, Mohammad Parwez
India, New Delhi
Institute of Genomics and Integrative Biology India
United States, Tallahassee
Florida State University
Patowary, Ashok
India, New Delhi
Institute of Genomics and Integrative Biology India
Sarkar, Maharnob
India, New Delhi
Institute of Genomics and Integrative Biology India
Ali, Tamer
India, New Delhi
Institute of Genomics and Integrative Biology India
Egypt, Benha
Faculty of Science
Gupta, Shivani
India, New Delhi
Institute of Genomics and Integrative Biology India
Garg, Manali
India, New Delhi
Institute of Genomics and Integrative Biology India
Singh, Meghna
India, New Delhi
Institute of Genomics and Integrative Biology India
Purkanti, Ramya
India, New Delhi
Institute of Genomics and Integrative Biology India
Scaria, Vinod
India, New Delhi
Institute of Genomics and Integrative Biology India
Sivasubbu, Sridhar
India, New Delhi
Institute of Genomics and Integrative Biology India
Brahmachari, Vani
India, New Delhi
Dr. B.r. Ambedkar Center for Biomedical Research
Beena Pillai,
India, New Delhi
Institute of Genomics and Integrative Biology India
Statistics
Citations: 46
Authors: 13
Affiliations: 4
Identifiers
Doi:
10.1093/nar/gks665
ISSN:
03051048
e-ISSN:
13624962
Research Areas
Genetics And Genomics