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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Phenotypic, genomic, and transcriptional characterization of Streptococcus pneumoniae interacting with human pharyngeal cells
BMC Genomics, Volume 14, No. 1, Article 383, Year 2013
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Description
Background: Streptococcus pneumoniae is a leading cause of childhood morbidity and mortality worldwide, despite the availability of effective pneumococcal vaccines. Understanding the molecular interactions between the bacterium and the host will contribute to the control and prevention of pneumococcal disease.Results: We used a combination of adherence assays, mutagenesis and functional genomics to identify novel factors involved in adherence. By contrasting these processes in two pneumococcal strains, TIGR4 and G54, we showed that adherence and invasion capacities vary markedly by strain. Electron microscopy showed more adherent bacteria in association with membranous pseudopodia in the TIGR4 strain. Operons for cell wall phosphorylcholine incorporation (lic), manganese transport (psa) and phosphate utilization (phn) were up-regulated in both strains on exposure to epithelial cells. Pneumolysin, pili, stress protection genes (adhC-czcD) and genes of the type II fatty acid synthesis pathway were highly expressed in the naturally more invasive strain, TIGR4. Deletion mutagenesis of five gene regions identified as regulated in this study revealed attenuation in adherence. Most strikingly, {increment}SP_1922 which was predicted to contain a B-cell epitope and revealed significant attenuation in adherence, appeared to be expressed as a part of an operon that includes the gene encoding the cytoplasmic pore-forming toxin and vaccine candidate, pneumolysin.Conclusion: This work identifies a list of novel potential pneumococcal adherence determinants. © 2013 Kimaro Mlacha et al.; licensee BioMed Central Ltd.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3708772/bin/1471-2164-14-383-S1.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3708772/bin/1471-2164-14-383-S2.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3708772/bin/1471-2164-14-383-S3.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3708772/bin/1471-2164-14-383-S4.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3708772/bin/1471-2164-14-383-S5.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3708772/bin/1471-2164-14-383-S6.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3708772/bin/1471-2164-14-383-S7.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3708772/bin/1471-2164-14-383-S8.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3708772/bin/1471-2164-14-383-S9.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3708772/bin/1471-2164-14-383-S10.xls
https://efashare.b-cdn.net/share/pmc/articles/PMC3708772/bin/1471-2164-14-383-S11.pdf
Authors & Co-Authors
Kimaro-Mlacha, Sheila Z.
Kenya, Nairobi
Wellcome Trust Research Laboratories Nairobi
United States, Baltimore
University of Maryland School of Medicine
South Africa, Johannesburg
University of the Witwatersrand
Romero-Steiner, Sandra
United States, Atlanta
Centers for Disease Control and Prevention
Dunning Hotopp, Julie C.
United States, Baltimore
University of Maryland School of Medicine
Kumar, Nikhil Ram
United States, Baltimore
University of Maryland School of Medicine
Ishmael, Nadeeza
United States, Baltimore
University of Maryland School of Medicine
Riley, David R.
United States, Baltimore
University of Maryland School of Medicine
Farooq, Umar
United States, Baltimore
University of Maryland School of Medicine
Creasy, Todd H.
United States, Baltimore
University of Maryland School of Medicine
Tallon, Luke J.
United States, Baltimore
University of Maryland School of Medicine
Liu, Xinyue
United States, Baltimore
University of Maryland School of Medicine
Goldsmith, Cynthia S.
United States, Atlanta
National Center for Emerging and Zoonotic Infectious Diseases
Sampson, Jacquelyn S.
United States, Atlanta
Centers for Disease Control and Prevention
Carlone, George M.
United States, Atlanta
Centers for Disease Control and Prevention
Hollingshead, Susan K.
United States, Birmingham
The University of Alabama at Birmingham
Scott, John Anthony Gerard
Kenya, Nairobi
Wellcome Trust Research Laboratories Nairobi
United Kingdom, Oxford
Nuffield Department of Medicine
Tettelin, Hervé
United States, Baltimore
University of Maryland School of Medicine
Statistics
Citations: 16
Authors: 16
Affiliations: 7
Identifiers
Doi:
10.1186/1471-2164-14-383
e-ISSN:
14712164
Research Areas
Cancer
Genetics And Genomics
Maternal And Child Health