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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Excellent laundry detergent compatibility and high dehairing ability of the Bacillus pumilus CBS alkaline proteinase (SAPB)
Biotechnology and Bioprocess Engineering, Volume 14, No. 4, Year 2009
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Description
The newly Tunisian soil-isolated bacterium, producing the alkaline proteinase termed SAPB that was already purified and characterized [1], was assigned as Bacillus pumilus CBS strain on the basis of biochemical properties and 16S rRNA gene sequencing. The maximum protease activity recorded after 24 h of incubation in an optimized medium at 37°C was 6,500 U/mL in shaking flask culture and 25,000 U/mL in fermentor. SAPB showed excellent stability and compatibility in laundry detergent retaining more than 98% of its initial activity after pre-incubation for 1 h at 40°C with Det, followed by OMO (97%), Dinol (94%), and Dixan (93%). Examination of various stained cloth pieces exhibited a remarkable efficiency in the removal of blood and chocolate stains. More interestingly, SAPB demonstrated powerful dehairing capabilities of hair removal from skin with minimal damage on the collagen and a nearly complete feather-degradation. Likewise, Bacillus pumilus CBS effectively degraded feather-meal (98.5%), chicken feather (92%), goat hair (80%), and bovine hair (68%) whereas sheep wool under went less degradation. Keratin-degradation resulted in sulfhdryl group formation (0.95-3.91 μM). © The Korean Society for Biotechnology and Bioengineering and Springer Berlin Heidelberg 2009.
Authors & Co-Authors
Jaouadi, Bassem
Tunisia, Sfax
Centre de Biotechnologie de Sfax
Ellouz-Chaabouni, Samia
Tunisia, Sfax
Ecole Nationale D'ingénieurs de Sfax
Ben Ali, Mamdouh
Tunisia, Sfax
Centre de Biotechnologie de Sfax
Messaoud, Ezzedine Ben
Tunisia, Sfax
Centre de Biotechnologie de Sfax
Naili, Belgacem
Tunisia, Sfax
Centre de Biotechnologie de Sfax
Dhouib, Abdelhafidh
Tunisia, Sfax
Centre de Biotechnologie de Sfax
Bejar, S.
Tunisia, Sfax
Centre de Biotechnologie de Sfax
Statistics
Citations: 69
Authors: 7
Affiliations: 2
Identifiers
Doi:
10.1007/s12257-008-0244-8
ISSN:
12268372
Research Areas
Genetics And Genomics