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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Increased peritoneal and endometrial gene expression of biologically relevant cytokines and growth factors during the menstrual phase in women with endometriosis
Fertility and Sterility, Volume 85, No. 6, Year 2006
Notification
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Description
Objective: To examine differential messenger RNA (mRNA) expression of relevant cytokines, metalloproteases, growth and adhesion factors in endometrium and peritoneum from women with endometriosis when compared with women without the disease during menstrual and luteal phases of the cycle. Design: Patients with endometriosis were compared with control patients. Setting: University hospital. Patient(s): A total of 35 patients (20 patients during the luteal phase and 15 patients during the menstrual phase) were selected for this study on the basis of cycle phase and presence or absence of endometriosis. Intervention(s): In this study, endometriosis was laparoscopically and histologically confirmed in 24 women with endometriosis of revised American Society for Reproductive Medicine (ASRM) stage I-II (n = 12) and revised ASRM stage III-IV (n = 12), and the presence of a normal pelvis was documented by laparoscopy in 11 control patients. The macroscopically normal peritoneum tissues were collected from lateral wall left or right, near the colon ascendens or descendens. Main Outcome Measure(s): The expression levels were determined as ratios between the target molecules and β-actin as housekeeping gene. Result(s): In women with endometriosis, peritoneal mRNA levels of matrix metalloproteinase (MMP)-3, transforming growth factor-β, interleukin (IL)-6, and intercellular adhesion molecule-1 and endometrial mRNA levels of MMP-3, tumor necrosis factor (TNF)-α, and IL-8 were significantly higher during the menstrual phase when compared with luteal phase. During the menstrual phase of the cycle, both endometrial expression of TNF-α, IL-8, and MMP-3 mRNA levels and peritoneal expression of transforming growth factor-β, IL-6, and intercellular adhesion molecule-1 mRNA levels were significantly higher in women with endometriosis when compared with controls. Immunohistochemical staining confirmed the presence of TNF-α in peritoneum and endometrium in both women with endometriosis and controls. Conclusion(s): Increased endometrial and peritoneal cytokine mRNA expression during menstruation may contribute to a pelvic inflammatory microenvironment favoring the development of endometriosis. © 2006 American Society for Reproductive Medicine.
Authors & Co-Authors
Kyama, Cleophas Mutinda
Belgium, Leuven
Ku Leuven
Kenya, Nairobi
National Museums of Kenya
Overbergh, Lutgart
Belgium, Leuven
Ku Leuven– University Hospital Leuven
Debrock, Sophie
Belgium, Leuven
Ku Leuven
Valckx, Dirk
Belgium, Leuven
Ku Leuven– University Hospital Leuven
Vander Perre, Sarah
Belgium, Leuven
Ku Leuven
Meuleman, Christel L.
Belgium, Leuven
Ku Leuven
Mihályi, Attila M.
Belgium, Leuven
Ku Leuven
Mwenda, Jason Mathiu
Kenya, Nairobi
National Museums of Kenya
Mathieu, Chantal
Belgium, Leuven
Ku Leuven– University Hospital Leuven
D'Hooghe, Thomas Maria
Belgium, Leuven
Ku Leuven
Kenya, Nairobi
National Museums of Kenya
Statistics
Citations: 144
Authors: 10
Affiliations: 3
Identifiers
Doi:
10.1016/j.fertnstert.2005.11.060
ISSN:
00150282
Research Areas
Cancer
Genetics And Genomics
Health System And Policy
Sexual And Reproductive Health
Study Design
Randomised Control Trial
Participants Gender
Female