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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Evaluation of enhanced thermostability and operational stability of carbonic anhydrase from Micrococcus species
Applied Biochemistry and Biotechnology, Volume 170, No. 4, Year 2013
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Description
Carbonic anhydrase (CA) was purified from Micrococcus lylae and Micrococcus luteus with 49.90 and 53.8 % yield, respectively, isolated from calcium carbonate kilns. CA from M. lylae retained 80 % stability in the pH and temperature range of 6.0-8.0 and 35-45 C, respectively. However, CA from M. luteus was stable in the pH and temperature range of 7.5-10.0 and 35-55 C, respectively. Cross-linked enzyme aggregates (CLEAs) raised the transition temperature of M. lylae and M. luteus CA up to 67.5 and 74.0 C, while the operational stability (T 1/2) of CA at 55 C was calculated to be 7.7 and 12.0 h, respectively. CA from both the strains was found to be monomeric in nature with subunit molecular weight and molecular mass of 29 kDa. Ethoxozolamide was identified as the most potent inhibitor based on both IC 50 values and inhibitory constant measurement (K i). The K m and V max for M. lylae CA (2.31 mM; 769.23 μmol/mg/min) and M. luteus CA (2.0 mM; 1,000 μmol/mg/min) were calculated from Lineweaver-Burk plots in terms of esterase activity. Enhanced thermostability of CLEAs alleviates its role in operational stability for application at an on-site scrubber. The characteristic profile of purified CA from Micrococcus spp. advocates its effective application in biomimetic CO 2 sequestration. © 2013 Springer Science+Business Media New York.
Authors & Co-Authors
Bhattacharya, Abhishek
India, Jabalpur
Rani Durgavati Vishwavidyalaya
South Africa, Grahamstown
Rhodes University
Shrivastava, Ankita
India, Jabalpur
Rani Durgavati Vishwavidyalaya
Sharma, Anjana
India, Jabalpur
Rani Durgavati Vishwavidyalaya
Statistics
Citations: 17
Authors: 3
Affiliations: 2
Identifiers
Doi:
10.1007/s12010-013-0226-y
ISSN:
02732289
e-ISSN:
15590291