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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
The chemotherapeutic agents nocodazole and amsacrine cause meiotic delay and non-disjunction in spermatocytes of mice
Mutation Research - Genetic Toxicology and Environmental Mutagenesis, Volume 651, No. 1-2, Year 2008
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Description
Aneuploidy of germ cells contributes to reduced fertility, foetal wastage and genetic defects. The possible risk of aneuploidy induction by the cancer chemotherapeutic drugs amsacrine (AMSA) and nocodazole (NOC) was investigated in male mice. Two molecular cytogenetic approaches were used: (1) the BrdU-incorporation assay to test the altered duration of meiotic divisions and (2) the sperm-FISH assay to determine aneuploidy induction during meiosis by observing hyperhaploid and diploid sperm. Sperm were sampled from the Caudae epididymes of treated and solvent control males. Single intraperitoneal injections with NOC (35 mg/kg) and AMSA (15 mg/kg) caused a meiotic delay of 24 h. The timing of sperm sampling for the sperm-FISH assay was adjusted accordingly, i.e. 23 days after treatment. Mice were treated with 18, 35 and 50 mg/kg of NOC, or 5, 10, 15 and 20 mg/kg of AMSA. Significant dose-dependent increases above the concurrent controls in the frequencies of hyperhaploid sperm were found with both agents. Significant increases in the frequencies of diploid sperm were found only with AMSA. These results provide a basis for genetic counselling of patients under AMSA or NOC chemotherapy. During a period of 3-4 months after the end of chemotherapy, they may stand a higher risk of siring chromosomally abnormal offspring. © 2007 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Attia, Sabry Mohamed
Egypt, Cairo
Faculty of Pharmacy
Germany, Oberschleissheim
Helmholtz Center Munich German Research Center for Environmental Health
Badary, Osama Ahmed
Egypt, Helwan
Faculty of Pharmacy
Hamada, Farid Mohamed Ahmed
Egypt, Cairo
Faculty of Pharmacy
de Angelis, Martin Hrabé
Germany, Oberschleissheim
Helmholtz Center Munich German Research Center for Environmental Health
Adler, Ilse Dore
Germany, Oberschleissheim
Helmholtz Center Munich German Research Center for Environmental Health
Statistics
Citations: 25
Authors: 5
Affiliations: 3
Identifiers
Doi:
10.1016/j.mrgentox.2007.10.011
ISSN:
13835718
Research Areas
Cancer
Genetics And Genomics
Sexual And Reproductive Health
Participants Gender
Male