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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Genotyping and molecular characterization of hepatitis B virus in liver disease patients in Kenya
Infection, Genetics and Evolution, Volume 20, Year 2013
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Description
Hepatitis B virus (HBV) genotypes are important in both the clinical manifestation of disease and treatment response. Although Kenya belongs to the African Region (AFR-E) characterized by high mortality and hyperendemicity of HBV, there is a paucity of HBV genotyping data. The aim of this study was to molecularly characterize the basic core promoter/precore (BCP/PC) and complete surface (S) regions of HBV isolated from 61 HBsAg-positive liver disease patients attending Kenyatta National Hospital in Nairobi. HBsAg, HBeAg and viral loads were determined. HBV DNA was amplified and sequenced from 58/61 patients. In addition to the complete genome of two isolates, the BCP/PC and the complete S regions of 43 and 38 isolates, respectively were sequenced. Following phylogenetic analysis of the S region, 38 isolates clustered with subgenotype A1, whereas two isolates clustered with genotype D, one with subgenotype D1 and another as an outlier of the clade containing subgenotype D6 and the D/E recombinant. When the complete genome of the latter isolate was sequenced it clustered with D6. The majority of isolates belonged to serological subtype adw2 and only four to ayw2. Three distinct groups of subgenotype A1, distinguished by different amino acid motifs, circulate in Kenya: two in the African cluster and a monophyletic clade in the "Asian" cluster. HBeAg-negativity was a result of G1896A in genotype D isolates, whereas in subgenotype A1, the HBeAg-negativity was a result of mutations in the Kozak region (1809-1812) or precore start codon (1814-1816). Mutations at positions 1762 and 1764 occurred more frequently in HCC patients (p<. 0.05). In conclusion, subgenotypes A1, D1 and D6 circulate in liver disease patients in Kenya, with A1 predominating. © 2013 Elsevier B.V.
Authors & Co-Authors
Ochwoto, Missiani
South Africa, Johannesburg
University of the Witwatersrand
Kenya, Nairobi
Kenya Medical Research Institute
Kenya, Nairobi
Jomo Kenyatta University of Agriculture and Technology
Chauhan, Ranjit
South Africa, Johannesburg
University of the Witwatersrand
Gopalakrishnan, Deepak
South Africa, Johannesburg
University of the Witwatersrand
Chen, Chien Yu
South Africa, Johannesburg
University of the Witwatersrand
Ng'Ang'A, Zipporah W.
Kenya, Nairobi
Jomo Kenyatta University of Agriculture and Technology
Okoth, Frederick A.
Kenya, Nairobi
Kenya Medical Research Institute
Kioko, Henry M.
Kenya, Nairobi
Kenyatta National Hospital
Kimotho, James Hungo
Kenya, Nairobi
Kenya Medical Research Institute
Kaiguri, Peter
Kenya, Nairobi
Kenya Medical Research Institute
Kramvis, Anna
South Africa, Johannesburg
University of the Witwatersrand
Statistics
Citations: 41
Authors: 10
Affiliations: 4
Identifiers
Doi:
10.1016/j.meegid.2013.08.013
ISSN:
15671348
e-ISSN:
15677257
Research Areas
Genetics And Genomics
Health System And Policy
Infectious Diseases
Study Locations
Kenya