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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Comparison of whole blood cultures and peripheral blood mononuclear cell cultures for evaluation of lymphocyte reactivity during chronic schistosomiasis
Transactions of the Royal Society of Tropical Medicine and Hygiene, Volume 75, No. 6, Year 1981
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Description
Lymphocyte blastogenesis during chronic schistosomiasis has been evaluated previously by either whole blood or peripheral blood mononuclear cell (PBMN) culture techniques. These two methods were compared in the present study. Blastogenesis to the mitogen phytohaemagglutinin was greater in the whole blood cultures at the time assayed. Substantial antigen-induced lymphocyte transformation was elicited in both culture systems using cells from uninfected patients. However, patients’ responses to schistosome-derived antigenic preparations as determined by whole blood culture were greatly reduced compared with PBMN cultures. In view of the known immunoregulatory activities associated with sera and cells from infected patients the whole blood technique may better reflect in vivo lymphocyte reactivity. The PBMN cultures are better suited to examine the immunoregulatory activities which may develop during this chronic infection. © 1981 The University of Chicago Press.
Authors & Co-Authors
Todd, C. W.
United States, Nashville
Vanderbilt University
Todd, C. W.
United States, Boston
Harvard T.h. Chan School of Public Health
Colley, Daniel G.
United States, Nashville
Vanderbilt University
Ramzy, Reda M.R.
Egypt
Bilharziasis Research Project
Habib, M.
Egypt
Bilharziasis Research Project
El-Alamy, Mohamed A.
Egypt
Bilharziasis Research Project
Statistics
Citations: 6
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.1016/0035-9203(81)90412-0
ISSN:
00359203
e-ISSN:
18783503
Research Areas
Infectious Diseases