Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Cell adhesion and focal contact formation on linear RGD molecular gradients: Study of non-linear concentration dependence effects
Nanomedicine: Nanotechnology, Biology, and Medicine, Volume 8, No. 4, Year 2012
Notification
URL copied to clipboard!
Description
Cell adhesion onto bioengineered surfaces is affected by a number of variables, including the former substrate derivatization process. In this investigation, we studied the correlation between cell adhesion and cell-adhesive ligand surface concentration and organization due to substrate modification. For this purpose, Arg-Gly-Asp (RGD) gradient surfaces were created on poly(methyl methacrylate) substrates by continuous hydrolysis and were then grafted with biotin-PEG-RGD molecules. Cell culture showed that adhesion behavior changes in a nonlinear way in the narrow range of RGD surface densities assayed (2.8 to 4.4 pmol/cm 2), with a threshold value of 4.0 pmol/cm 2 for successful cell attachment and spreading. This nonlinear dependence may be explained by nonhomogeneous RGD surface distribution at the nanometre scale, conditioned by the stochastic nature of the hydrolysis process. Atomic force microscopy analysis of the gradient surface showed an evolution of surface morphology compatible with this hypothesis. From the Clinical Editor: The authors observed by AFM nonlinear dependence of cell adhesion on RGD gradient surfaces with different surface densities. The nonlinear characteristics may be explained by non-homogeneous RGD surface distribution at the nanometer scale, conditioned by the stochastic nature of the hydrolysis process. © 2012 Elsevier Inc.
Authors & Co-Authors
Lagunas, Anna
Spain, Madrid
Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina
Spain, Barcelona
Instituto de Bioingeniería de Cataluña
Martínez, Elena M.
Spain, Madrid
Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina
Spain, Barcelona
Instituto de Bioingeniería de Cataluña
Prats-Alfonso, Elisabet
Spain, Madrid
Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina
Spain, Barcelona
Irb Barcelona - Institute for Research in Biomedicine
Acosta, Gerardo A.
Spain, Madrid
Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina
Spain, Barcelona
Irb Barcelona - Institute for Research in Biomedicine
Alberício, Fernando
Spain, Madrid
Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina
Spain, Barcelona
Irb Barcelona - Institute for Research in Biomedicine
Spain, Barcelona
Universitat de Barcelona
Samitier, Josep
Spain, Madrid
Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina
Spain, Barcelona
Instituto de Bioingeniería de Cataluña
Spain, Barcelona
Universitat de Barcelona
Statistics
Citations: 43
Authors: 6
Affiliations: 4
Identifiers
Doi:
10.1016/j.nano.2011.08.001
ISSN:
15499642