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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
environmental science
Bacterial diversity of field-caught mosquitoes, Aedes albopictus and Aedes aegypti, from different geographic regions of Madagascar
FEMS Microbiology Ecology, Volume 75, No. 3, Year 2011
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Description
Symbiotic bacteria are known to play important roles in the biology of insects, but the current knowledge of bacterial communities associated with mosquitoes is very limited and consequently their contribution to host behaviors is mostly unknown. In this study, we explored the composition and diversity of mosquito-associated bacteria in relation with mosquitoes' habitats. Wild Aedes albopictus and Aedes aegypti were collected in three different geographic regions of Madagascar. Culturing methods and denaturing gradient gel electrophoresis (DGGE) and sequencing of the rrs amplicons revealed that Proteobacteria and Firmicutes were the major phyla. Isolated bacterial genera were dominated by Bacillus, followed by Acinetobacter, Agrobacterium and Enterobacter. Common DGGE bands belonged to Acinetobacter, Asaia, Delftia, Pseudomonas, Enterobacteriaceae and an uncultured Gammaproteobacterium. Double infection by maternally inherited Wolbachia pipientis prevailed in 98% of males (n=272) and 99% of females (n=413); few individuals were found to be monoinfected with Wolbachia wAlbB strain. Bacterial diversity (Shannon-Weaver and Simpson indices) differed significantly per habitat whereas evenness (Pielou index) was similar. Overall, the bacterial composition and diversity were influenced both by the sex of individuals and by the environment inhabited by the mosquitoes; the latter might be related to both the vegetation and the animal host populations that Aedes used as food sources. © 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Authors & Co-Authors
Zouache, Karima
France, Lyon
Université de Lyon
France, Villeurbanne
Ecologie Microbienne Lem
Raharimalala, Fara Nantenaina
France, Lyon
Université de Lyon
France, Villeurbanne
Ecologie Microbienne Lem
Madagascar
Département D'entomologie de la Faculté Des Sciences D'antananarivo
Raquin, Vincent
France, Lyon
Université de Lyon
France, Villeurbanne
Ecologie Microbienne Lem
Trân van, Vân
France, Lyon
Université de Lyon
France, Villeurbanne
Ecologie Microbienne Lem
Raveloson, Lala Harivelo Ravaomanarivo
Madagascar
Département D'entomologie de la Faculté Des Sciences D'antananarivo
Ravelonandro, Pierre Hervé
Madagascar, Antananarivo
Centre National de Recherche Sur L’environnement
Mavingui, Patrick
France, Lyon
Université de Lyon
France, Villeurbanne
Ecologie Microbienne Lem
Statistics
Citations: 202
Authors: 7
Affiliations: 4
Identifiers
Doi:
10.1111/j.1574-6941.2010.01012.x
ISSN:
01686496
e-ISSN:
15746941
Research Areas
Food Security
Study Locations
Madagascar
Participants Gender
Female