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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Influence of hepatitis B virus genotypes on the intra- and extracellular expression of viral DNA and antigens
Hepatology, Volume 44, No. 4, Year 2006
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Description
Various genotypes of the hepatitis B virus (HBV) induce liver disease of distinct severity, but the underlying virological differences are not well defined. Huh7 cells were transfected with plasmids carrying 1.24-fold the HBV genome of different genotypes/subgenotypes (2 strains each for Aa/A1, Ae/A2, Ba/B2 and D; 3 each for Bj/B1 and C). HBV DNA levels in cell lysates, determined by Southern hybridization, were the highest for C followed by Bj/Ba and D/Ae (P < .01), and the lowest for Aa (P < .01), whereas in culture media, they were the highest for Bj, distantly followed by Ba/C/D and further by Ae/Aa (P < .01). The intracellular expression of core protein was more than 3-fold lower for Ae/Aa than the others. Hepatitis B e antigen (HBeAg) was excreted in a trend similar to that of HBV DNA with smaller differences. Secretion of hepatitis B surface antigen (HBsAg) was most abundant for Ae followed by Aa, Ba, Bj/C and remotely by D, which was consistent with mRNA levels. Cellular stress determined by the reporter assay for Grp78 promoter was higher for C and Ba than the other genotypes/subgenotypes (P < .01). Severe combined immunodeficiency mice transgenic for urokinase-type plasminogen activator (uPA/SCID), with the liver replaced for human hepatocytes, were inoculated with virions passed in mouse and recovered from culture supernatants. HBV DNA levels in their sera were higher for C than Ae by 2 logs during 4-7 weeks after inoculation. In conclusion, virological differences among HBV genotypes were demonstrated both in vitro and in vivo. These differences may influence HBV infections with distinct genotypes in clinical and epidemiological settings. Copyright © 2006 by the American Association for the Study of Liver Diseases.
Authors & Co-Authors
Sugiyama, Massaya
Japan, Nagoya
Nagoya City University Graduate School of Medical Sciences
Tanaka, Yasuhito
Japan, Nagoya
Nagoya City University Graduate School of Medical Sciences
Kato, Takanobu
Japan, Nagoya
Nagoya City University Graduate School of Medical Sciences
Orito, Etsurou
Japan, Nagoya
Nagoya City University Graduate School of Medical Sciences
Ito, Kiyoaki
Japan, Nagoya
Nagoya City University Graduate School of Medical Sciences
Acharya, Subrat Kumar
India, New Delhi
All India Institute of Medical Sciences, new Delhi
Gish, Robert G.
United States, San Francisco
California Pacific Medical Center
Kramvis, Anna
South Africa, Tygerberg
South African Medical Research Council
Shimada, Takashi
Japan, Higashihiroshima
Phoenixbio Co., Ltd.
Izumi, Namiki
Japan, Musashino
Japanese Red Cross Musashino Hospital
Kaito, Masahiko
Japan, Tsu
Mie University Faculty of Medicine
Miyakawa, Yuzo
Japan, Tokyo
Miyakawa Memorial Research Foundation
Mizokami, Masashi
Japan, Nagoya
Nagoya City University Graduate School of Medical Sciences
Statistics
Citations: 281
Authors: 13
Affiliations: 8
Identifiers
Doi:
10.1002/hep.21345
ISSN:
02709139
Research Areas
Genetics And Genomics
Infectious Diseases