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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
rKLO8, a Novel Leishmania donovani - Derived Recombinant Immunodominant Protein for Sensitive Detection of Visceral Leishmaniasis in Sudan
PLoS Neglected Tropical Diseases, Volume 7, No. 7, Article e2322, Year 2013
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Description
Background: For effective control of visceral leishmaniasis (VL) in East Africa, new rapid diagnostic tests are required to replace current tests with low sensitivity. The aim of this study is to improve diagnosis of VL in East Africa by testing a new antigen from an autochthonous L. donovani strain in Sudan. Methodology and Principle Findings: We cloned, expressed and purified a novel recombinant protein antigen of L. donovani from Sudan, designated rKLO8, that contains putative conserved domains with significant similarity to the immunodominant kinesin proteins of Leishmania. rKLO8 exhibited 93% and 88% amino acid identity with cloned kinesin proteins of L. infantum (synonymous L. chagasi) (K39) and L. donovani (KE16), respectively. We evaluated the diagnostic efficiency of the recombinant protein in ELISA for specific detection of VL patients from Sudan. Data were compared with a rK39 ELISA and two commercial kits, the rK39 strip test and the direct agglutination test (DAT). Of 106 parasitologically confirmed VL sera, 104 (98.1%) were tested positive by rKLO8 as compared to 102 (96.2%) by rK39. Importantly, the patients' sera showed increased reactivity with rKLO8 than rK39. Specificity was 96.1% and 94.8% for rKLO8- and rK39 ELISAs, respectively. DAT showed 100% specificity and 94.3% sensitivity while rK39 strip test performed with 81.1% sensitivity and 98.7% specificity. Conclusion: The increased reactivity of Sudanese VL sera with the rKLO8 makes this antigen a potential candidate for diagnosis of visceral leishmaniasis in Sudan. However, the suitability at the field level will depend on its performance in a rapid test format. © 2013 Abass et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3715527/bin/pntd.0002322.s001.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC3715527/bin/pntd.0002322.s002.doc
Authors & Co-Authors
Abass, Elfadil Mustafa
Germany, Marburg
Philipps-universität Marburg
Sudan, Omdurman
Ahfad University for Women
Bollig, Nadine
Germany, Marburg
Philipps-universität Marburg
Reinhard, Katharina
Germany, Marburg
Philipps-universität Marburg
Camara, Bärbel
Germany, Marburg
Philipps-universität Marburg
Mansour, Durria
Sudan, Omdurman
Ahfad University for Women
Visekruna, Alexander
Germany, Marburg
Philipps-universität Marburg
Lohoff, Michael D.
Germany, Marburg
Philipps-universität Marburg
Steinhoff, Ulrich
Germany, Marburg
Philipps-universität Marburg
Statistics
Citations: 56
Authors: 8
Affiliations: 2
Identifiers
Doi:
10.1371/journal.pntd.0002322
ISSN:
19352727
e-ISSN:
19352735
Study Locations
Multi-countries
Sudan