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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Mechanotransduction of Ultrasound is Frequency Dependent Below the Cavitation Threshold
Ultrasound in Medicine and Biology, Volume 39, No. 7, Year 2013
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Description
This study provides evidence that low-intensity ultrasound directly affects nuclear processes, and the magnitude of the effect varies with frequency. In particular, we show that the transcriptional induction of first load-inducible genes, which is independent of new protein synthesis, is frequency dependent. Bovine chondrocytes were exposed to low-intensity (below the cavitational threshold) ultrasound at 2, 5 and 8 MHz. Ultrasound elevated the expression of early response genes c-Fos, c-Jun and c-Myc, maximized at 5 MHz. The phosphorylated ERK inhibitor PD98059 abrogated any increase in c-series gene expression, suggesting that signaling occurs via the MAPPK/ERK pathway. However, phosphorylated ERK levels did not change with ultrasound frequency, indicating that processes downstream of ERK phosphorylation (such as nuclear transport and chromatin reorganization) respond to ultrasound with frequency dependence. A quantitative, biphasic mathematical model based on Biot theory predicted that cytoplasmic and nuclear stress is maximized at 5.2 ± 0.8 MHz for a chondrocyte, confirming experimental measurements. © 2013 World Federation for Ultrasound in Medicine & Biology.
Authors & Co-Authors
Louw, Tobias M.
United States, Lincoln
University of Nebraska–lincoln
Viljoen, Hendrik J.
United States, Lincoln
University of Nebraska–lincoln
Subramanian, Anuradha
United States, Lincoln
University of Nebraska–lincoln
Statistics
Citations: 48
Authors: 3
Affiliations: 1
Identifiers
Doi:
10.1016/j.ultrasmedbio.2013.01.015
ISSN:
03015629
Research Areas
Cancer
Genetics And Genomics
Health System And Policy
Study Approach
Quantitative