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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Single-cell multi-omics analysis of the immune response in COVID-19
Nature Medicine, Volume 27, No. 5, Year 2021
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Description
Analysis of human blood immune cells provides insights into the coordinated response to viral infections such as severe acute respiratory syndrome coronavirus 2, which causes coronavirus disease 2019 (COVID-19). We performed single-cell transcriptome, surface proteome and T and B lymphocyte antigen receptor analyses of over 780,000 peripheral blood mononuclear cells from a cross-sectional cohort of 130 patients with varying severities of COVID-19. We identified expansion of nonclassical monocytes expressing complement transcripts (CD16+C1QA/B/C+) that sequester platelets and were predicted to replenish the alveolar macrophage pool in COVID-19. Early, uncommitted CD34+ hematopoietic stem/progenitor cells were primed toward megakaryopoiesis, accompanied by expanded megakaryocyte-committed progenitors and increased platelet activation. Clonally expanded CD8+ T cells and an increased ratio of CD8+ effector T cells to effector memory T cells characterized severe disease, while circulating follicular helper T cells accompanied mild disease. We observed a relative loss of IgA2 in symptomatic disease despite an overall expansion of plasmablasts and plasma cells. Our study highlights the coordinated immune response that contributes to COVID-19 pathogenesis and reveals discrete cellular components that can be targeted for therapy. © 2021, The Author(s).
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC8121667/bin/41591_2021_1329_MOESM1_ESM.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC8121667/bin/41591_2021_1329_MOESM2_ESM.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC8121667/bin/41591_2021_1329_MOESM3_ESM.xlsx
https://efashare.b-cdn.net/share/pmc/articles/PMC8121667/bin/41591_2021_1329_MOESM4_ESM.xlsx
Authors & Co-Authors
Morgan, Michael D.L.
United Kingdom, Hinxton
European Molecular Biology Laboratory
United Kingdom, Cambridge
University of Cambridge
Olabi, Bayanne
United Kingdom, Newcastle
Newcastle University
Prigmore, Elena
United Kingdom, Hinxton
Wellcome Sanger Institute
Gothe, Florian
United Kingdom, Newcastle
Newcastle University
Germany, Munich
Ludwig-maximilians-universität München
Payne, Rebecca P.
United Kingdom, Newcastle
Newcastle University
Bergamaschi, Laura
United Kingdom, Cambridge
University of Cambridge
Mescia, Federica
United Kingdom, Cambridge
University of Cambridge
Janes, Sam M.
United Kingdom, London
University College London
United Kingdom, London
University College London Hospitals Nhs Foundation Trust
Smith, Claire Mary
United Kingdom, London
Ucl Great Ormond Street Institute of Child Health
Wilson, Caroline L.
United Kingdom, Newcastle
Newcastle University
Coxhead, Jonathan M.
United Kingdom, Newcastle
Newcastle University
Bacardit, Jaume
United Kingdom, Newcastle
Newcastle University
Baker, Stephen G.
United Kingdom, Cambridge
University of Cambridge
Bradley, John Richard
United Kingdom, Cambridge
University of Cambridge
United Kingdom, Cambridge
Cambridge University Hospitals Nhs Foundation Trust
Dougan, Gordon J.
United Kingdom, Cambridge
University of Cambridge
Goodfellow, Ian Gordon
United Kingdom, Cambridge
Addenbrooke's Hospital
Gupta, Ravindra K.
United Kingdom, Cambridge
University of Cambridge
Hess, Christoph
United Kingdom, Cambridge
University of Cambridge
Switzerland, Basel
Universitätsspital Basel
Switzerland, Basel
Universitat Basel
Kingston, Nathalie
United Kingdom, Cambridge
Cambridge University Hospitals Nhs Foundation Trust
United Kingdom, Cambridge
University of Cambridge
Lehner, P. Joseph
United Kingdom, Cambridge
University of Cambridge
Matheson, Nicholas J.
United Kingdom, Cambridge
University of Cambridge
Saunders, Caroline
United Kingdom, Cambridge
Cambridge University Hospitals Nhs Foundation Trust
Smith, Kenneth G.C.
United Kingdom, Cambridge
University of Cambridge
Summers, Charlotte
United Kingdom, Cambridge
University of Cambridge
United Kingdom, Cambridge
Wellcome-mrc Institute of Metabolic Science
United Kingdom, Cambridge
Royal Papworth Hospital Nhs Foundation Trust
United Kingdom, Cambridge
Addenbrooke's Hospital
Toshner, Mark R.
United Kingdom, Cambridge
University of Cambridge
United Kingdom, Cambridge
Wellcome-mrc Institute of Metabolic Science
United Kingdom, Cambridge
Royal Papworth Hospital Nhs Foundation Trust
Weekes, Michael P.
Unknown Affiliation
Bucke, Ashlea
United Kingdom, Cambridge
Cambridge University Hospitals Nhs Foundation Trust
Calder, Jo
United Kingdom, Cambridge
Cambridge University Hospitals Nhs Foundation Trust
Canna, Laura
United Kingdom, Cambridge
Cambridge University Hospitals Nhs Foundation Trust
Domingo, Jason
United Kingdom, Cambridge
Cambridge University Hospitals Nhs Foundation Trust
Elmer, Anne
United Kingdom, Cambridge
Cambridge University Hospitals Nhs Foundation Trust
Harris, Julie
United Kingdom, Cambridge
University of Cambridge
Hewitt, Sarah
United Kingdom, Cambridge
Cambridge University Hospitals Nhs Foundation Trust
Kennet, Jane
United Kingdom, Cambridge
Cambridge University Hospitals Nhs Foundation Trust
Jose, Sherly
United Kingdom, Cambridge
Cambridge University Hospitals Nhs Foundation Trust
Kourampa, Jenny
United Kingdom, Cambridge
Cambridge University Hospitals Nhs Foundation Trust
Meadows, Anne
United Kingdom, Cambridge
Cambridge University Hospitals Nhs Foundation Trust
Price, Jane
United Kingdom, Cambridge
Cambridge University Hospitals Nhs Foundation Trust
Rastall, Rebecca J.
United Kingdom, Cambridge
Cambridge University Hospitals Nhs Foundation Trust
Ribeiro, Carla G.
United Kingdom, Cambridge
Cambridge University Hospitals Nhs Foundation Trust
Rowlands, Jane
United Kingdom, Cambridge
Cambridge University Hospitals Nhs Foundation Trust
Tordesillas, Hugo
United Kingdom, Cambridge
Cambridge University Hospitals Nhs Foundation Trust
Dunmore, Benjamin J.
United Kingdom, Cambridge
University of Cambridge
Huang, Christopher L.H.
United Kingdom, Cambridge
University of Cambridge
Huhn, Oisín
United Kingdom, Cambridge
Rosie Hospital
Jarvis, Isobel
United Kingdom, Cambridge
University of Cambridge
Turner, Lori
United Kingdom, Cambridge
University of Cambridge
Dewhurst, Eleanor F.
United Kingdom, Cambridge
Cambridge University Hospitals Nhs Foundation Trust
United Kingdom, Cambridge
University of Cambridge
Papadia, Sofia
United Kingdom, Cambridge
Cambridge University Hospitals Nhs Foundation Trust
United Kingdom, Cambridge
University of Cambridge
Stirrups, Kathleen E.
United Kingdom, Cambridge
Cambridge University Hospitals Nhs Foundation Trust
United Kingdom, Cambridge
University of Cambridge
Townsend, Paul A.
United Kingdom, Cambridge
Cambridge University Hospitals Nhs Foundation Trust
United Kingdom, Cambridge
University of Cambridge
Simpson, Alistair John
United Kingdom, Newcastle
Newcastle University
Hambleton, Sophie
United Kingdom, Newcastle
Newcastle University
Lyons, Paul A.
United Kingdom, Cambridge
University of Cambridge
Meyer, Kerstin B.
United Kingdom, Hinxton
Wellcome Sanger Institute
Nikolić, Marko Z.
United Kingdom, London
University College London
United Kingdom, London
University College London Hospitals Nhs Foundation Trust
Duncan, Christopher J.A.
United Kingdom, Newcastle
Newcastle University
United Kingdom, Newcastle
The Newcastle Upon Tyne Hospitals Nhs Foundation Trust
Teichmann, Sarah Amalia
United Kingdom, Hinxton
Wellcome Sanger Institute
United Kingdom, Cambridge
University of Cambridge
Clatworthy, Menna Ruth
United Kingdom, Cambridge
University of Cambridge
United Kingdom, Hinxton
Wellcome Sanger Institute
United Kingdom, Cambridge
Cambridge Institute of Therapeutic Immunology and Infectious Disease
Marioni, John Carlo
United Kingdom, Hinxton
European Molecular Biology Laboratory
United Kingdom, Cambridge
University of Cambridge
United Kingdom, Hinxton
Wellcome Sanger Institute
Haniffa, Muzlifah A.
United Kingdom, Newcastle
Newcastle University
United Kingdom, Hinxton
Wellcome Sanger Institute
United Kingdom, Newcastle
The Newcastle Upon Tyne Hospitals Nhs Foundation Trust
Statistics
Citations: 282
Authors: 61
Affiliations: 21
Identifiers
Doi:
10.1038/s41591-021-01329-2
ISSN:
10788956
Research Areas
Covid
Study Design
Cross Sectional Study
Cohort Study