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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
RNAi-mediated resistance to Cassava brown streak Uganda virus in transgenic cassava
Molecular Plant Pathology, Volume 12, No. 7, Year 2011
Notification
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Description
Cassava brown streak disease (CBSD), caused by Cassava brown streak Uganda virus (CBSUV) and Cassava brown streak virus (CBSV), is of new epidemic importance to cassava (Manihot esculenta Crantz) production in East Africa, and an emerging threat to the crop in Central and West Africa. This study demonstrates that at least one of these two ipomoviruses, CBSUV, can be efficiently controlled using RNA interference (RNAi) technology in cassava. An RNAi construct targeting the near full-length coat protein (FL-CP) of CBSUV was expressed constitutively as a hairpin construct in cassava. Transgenic cassava lines expressing small interfering RNAs (siRNAs) against this sequence showed 100% resistance to CBSUV across replicated graft inoculation experiments. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis showed the presence of CBSUV in leaves and some tuberous roots from challenged controls, but not in the same tissues from transgenic plants. This is the first demonstration of RNAi-mediated resistance to the ipomovirus CBSUV in cassava. © 2011 The Authors. Molecular Plant Pathology © 2011 BSPP and Blackwell Publishing Ltd.
Authors & Co-Authors
Yadav, J. S.
United States, St. Louis
Donald Danforth Plant Science Center
Ogwok, Emmanuel
United States, St. Louis
Donald Danforth Plant Science Center
Uganda, Kampala
National Crops Resources Research Institute
Wagaba, Henry
United States, St. Louis
Donald Danforth Plant Science Center
Uganda, Kampala
National Crops Resources Research Institute
Patil, Basavaprabhu L.
United States, St. Louis
Donald Danforth Plant Science Center
Bagewadi, B.
United States, St. Louis
Donald Danforth Plant Science Center
Alicai, Titus
Uganda, Kampala
National Crops Resources Research Institute
Gaitán-Solís, Eliana
United States, St. Louis
Donald Danforth Plant Science Center
Taylor, Nigel James
United States, St. Louis
Donald Danforth Plant Science Center
Fauquet, Claude Maurice
United States, St. Louis
Donald Danforth Plant Science Center
Statistics
Citations: 98
Authors: 9
Affiliations: 2
Identifiers
Doi:
10.1111/j.1364-3703.2010.00700.x
ISSN:
14646722
e-ISSN:
13643703
Study Locations
Multi-countries
Uganda