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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
A new polymorphic locus, D7S411, isolated by cloning from preparative pulse-field gels is close to the mutation causing cystic fibrosis
Genomics, Volume 6, No. 1, Year 1990
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Description
The mutation causing cystic fibrosis (CF) has been localized to the DNA sequence of 700 kb bounded by the loci identified by the markers pMP6d-9 (D7S399) and pJ3.11 (D7S8). A 560-kb fragment obtained after SacII digestion of DNA from a cell line containing this region of human chromosome 7 in a mouse background was separated using pulse-field gel electrophoresis and isolated from the gel. The DNA was digested with BamHI prior to cloning into λ EMBL3. Approximately 0.1% of the resulting clones contained human repetitive sequences, and 24 such recombinants were studied. Of these, 23 are on chromosome 7; 8 clones were duplicated, and of the 15 different recombinants, 7 are between MET and INT1L1, and a further 7 are between INT1L1 and pMP6d-9, leaving a single marker, pG2, which is between pMP6d-9 and pJ3.11. pG2 recognizes an RFLP with XbaI. A cosmid walk from pG2 has generated a further marker, H80, which recognizes an RFLP with PstI. This new locus (D7S411) divides the remaining region between the CF flanking markers, thereby making it more accessible to fine pulse-field mapping and allowing the precise localization of further clones to this region. Although it is not possible to position the CF locus unequivocally with respect to D7S411, both polymorphic markers at this locus exhibit low but significant linkage disequilibrium with CF, placing the emphasis for the search for the gene on the D7S399 to D7S411 interval of 250 kb. None of the cystic fibrosis families that previously showed recombination with either pJ3.11 or INT1L1 is informative at the D7S411 locus. © 1990.
Authors & Co-Authors
Ramsay, Michèle
United Kingdom, London
Imperial College London
Wainwright, Brandon J.
United Kingdom, London
Imperial College London
Farrall, Martin
United Kingdom, London
Imperial College London
Estivill, Xavier
United Kingdom, London
Imperial College London
Halford, Stephanie
United Kingdom, London
Imperial College London
Wicking, Carol A.
United Kingdom, London
Imperial College London
Lench, Nicholas J.
United Kingdom, London
Imperial College London
Féréc, Claude F.
Unknown Affiliation
Farndon, Peter A.
Unknown Affiliation
Stanier, Philip M.
United Kingdom, London
Imperial College London
Williamson, Roberts W.
United Kingdom, London
Imperial College London
Scambler, Peter James
United Kingdom, London
Imperial College London
Statistics
Citations: 9
Authors: 12
Affiliations: 1
Identifiers
Doi:
10.1016/0888-7543(90)90446-2
ISSN:
08887543
Research Areas
Cancer
Genetics And Genomics