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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Spontaneous mutations in the plasmodium falciparum sarcoplasmic/endoplasmic reticulum Ca
2+
-ATPase (PfATP6) gene among geographically widespread parasite populations unexposed to artemisinin-based combination therapies
Antimicrobial Agents and Chemotherapy, Volume 55, No. 1, Year 2011
Notification
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Description
Recent reports on the decline of the efficacy of artemisinin-based combination therapies (ACTs) indicate a serious threat to malaria control. The endoplasmic/sarcoplasmic reticulum Ca2+-ATPase ortholog of Plasmodium falciparum (PfSERCA) has been suggested to be the target of artemisinin and its derivatives. It is assumed that continuous artemisinin pressure will affect polymorphism of the PfSERCA gene (serca) if the protein is the target. Here, we investigated the polymorphism of serca in parasite populations unexposed to ACTs to obtain baseline information for the study of potential artemisinin-driven selection of resistant parasites. Analysis of 656 full-length sequences from 13 parasite populations in Africa, Asia, Oceania, and South America revealed 64 single nucleotide polymorphisms (SNPs), of which 43 were newly identified and 38 resulted in amino acid substitutions. No isolates showed L263E and S769N substitutions, which were reportedly associated with artemisinin resistance. Among the four continents, the number of SNPs was highest in Africa. In Africa, Asia, and Oceania, common SNPs, or those with a minor allele frequency of ≥0.05, were less prevalent, with most SNPs noted to be continent specific, whereas in South America, common SNPs were highly prevalent and often shared with those in Africa. Of 50 amino acid haplotypes observed, only one haplotype (3D7 sequence) was seen in all four continents (64%). Forty-eight haplotypes had frequencies of less than 5%, and 40 haplotypes were continent specific. The geographical difference in the diversity and distribution of serca SNPs and haplotypes lays the groundwork for assessing whether some artemisinin resistance-associated mutations and haplotypes are selected by ACTs. Copyright © 2011 American Society for Microbiology. All Rights Reserved.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3019649/bin/supp_55_1_94__index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC3019649/bin/supp_55_1_94__1.pdf
Authors & Co-Authors
Tanabe, Kazuyuki
Japan, Suita
Research Institute for Microbial Diseases
Zakeri, Sedigheh
Iran, Tehran
Pasteur Institute of Iran
Palacpac, Nirianne Marie Q.
Japan, Suita
Research Institute for Microbial Diseases
Afsharpad, Mandana
Iran, Tehran
Pasteur Institute of Iran
Randrianarivelojosia, Milijaona
Madagascar, Antananarivo
Institut Pasteur de Madagascar
Kaneko, Akira
Sweden, Stockholm
Karolinska Institutet
Marma, Aung Swi Prue
Bangladesh, Dhaka
Directorate General of Health Services
Horii, Toshihiro
Japan, Suita
Research Institute for Microbial Diseases
Mita, T.
Japan, Tokyo
Tokyo Women's Medical University
Statistics
Citations: 39
Authors: 9
Affiliations: 6
Identifiers
Doi:
10.1128/AAC.01156-10
ISSN:
00664804
e-ISSN:
10986596
Research Areas
Genetics And Genomics
Infectious Diseases