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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Overproduction, purification, crystallization and preliminary X-ray diffraction analysis of Trypanosoma brucei gambiense glycerol kinase
Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Volume 66, No. 3, Year 2010
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Description
In the bloodstream forms of human trypanosomes, glycerol kinase (GK; EC 2.7.1.30) is one of the nine glycosomally compartmentalized enzymes that are essential for energy metabolism. In this study, a recombinant Trypanosoma brucei gambiense GK (rTbgGK) with an N-terminal cleavable His6 tag was overexpressed, purified to homogeneity and crystallized by the sitting-drop vapour-diffusion method using PEG 400 as a precipitant. A complete X-ray diffraction data set to 2.75 Å resolution indicated that the crystals belonged to the orthorhombic space group P212121, with unit-cell parameters a = 63.84, b = 121.50, c = 154.59 Å. The presence of two rTbgGK molecules in the asymmetric unit gives a Matthews coefficient (VM) of 2.5 Å3 Da-1, corresponding to 50% solvent content. © 2010 International Union of Crystallography All rights reserved.
Authors & Co-Authors
Balogun, Emmanuel Oluwadare
Japan, Tokyo
Graduate School of Medicine
Nigeria, Zaria
Ahmadu Bello University
Inaoka, Daniel Ken
Japan, Tokyo
Graduate School of Medicine
Kido, Yasutoshi
Japan, Tokyo
Graduate School of Medicine
Shiba, Tomoo
Japan, Tokyo
Graduate School of Medicine
Nara, Takeshi
Japan, Tokyo
Juntendo University School of Medicine
Aoki, Takashi
Japan, Tokyo
Juntendo University School of Medicine
Honma, Teruki
Japan, Wako
Riken
Tanaka, Akiko
Japan, Wako
Riken
Inoue, Masayuki
Japan, Tokyo
The University of Tokyo
Matsuoka, Shigeru
Japan, Tokyo
The University of Tokyo
Michels, Paul A.M.
Belgium, Brussels
De Duve Institute
Harada, Shigeharu
Japan, Kyoto
Kyoto Institute of Technology
Kita, Kiyoshi
Japan, Tokyo
Graduate School of Medicine
Statistics
Citations: 13
Authors: 13
Affiliations: 7
Identifiers
Doi:
10.1107/S1744309110000369
ISSN:
17443091
e-ISSN:
17443091