Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
High level secretion of cellobiohydrolases by Saccharomyces cerevisiae
Biotechnology for Biofuels, Volume 4, Article 30, Year 2011
Notification
URL copied to clipboard!
Description
Background: The main technological impediment to widespread utilization of lignocellulose for the production of fuels and chemicals is the lack of low-cost technologies to overcome its recalcitrance. Organisms that hydrolyze lignocellulose and produce a valuable product such as ethanol at a high rate and titer could significantly reduce the costs of biomass conversion technologies, and will allow separate conversion steps to be combined in a consolidated bioprocess (CBP). Development of Saccharomyces cerevisiae for CBP requires the high level secretion of cellulases, particularly cellobiohydrolases. Results: We expressed various cellobiohydrolases to identify enzymes that were efficiently secreted by S. cerevisiae. For enhanced cellulose hydrolysis, we engineered bimodular derivatives of a well secreted enzyme that naturally lacks the carbohydrate-binding module, and constructed strains expressing combinations of cbh1 and cbh2 genes. Though there was significant variability in the enzyme levels produced, up to approximately 0.3 g/L CBH1 and approximately 1 g/L CBH2 could be produced in high cell density fermentations. Furthermore, we could show activation of the unfolded protein response as a result of cellobiohydrolase production. Finally, we report fermentation of microcrystalline cellulose (Avicel) to ethanol by CBH-producing S. cerevisiae strains with the addition of beta-glucosidase. Conclusions: Gene or protein specific features and compatibility with the host are important for efficient cellobiohydrolase secretion in yeast. The present work demonstrated that production of both CBH1 and CBH2 could be improved to levels where the barrier to CBH sufficiency in the hydrolysis of cellulose was overcome. © 2011 Ilmén et al; licensee BioMed Central Ltd.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3224389/bin/1754-6834-4-30-S1.PDF
https://efashare.b-cdn.net/share/pmc/articles/PMC3224389/bin/1754-6834-4-30-S2.PDF
https://efashare.b-cdn.net/share/pmc/articles/PMC3224389/bin/1754-6834-4-30-S3.PDF
https://efashare.b-cdn.net/share/pmc/articles/PMC3224389/bin/1754-6834-4-30-S4.PDF
https://efashare.b-cdn.net/share/pmc/articles/PMC3224389/bin/1754-6834-4-30-S5.DOC
https://efashare.b-cdn.net/share/pmc/articles/PMC3224389/bin/1754-6834-4-30-S6.PDF
Authors & Co-Authors
Ilmén, Marja
Finland, Espoo
Teknologian Tutkimuskeskus Vtt
Den Haan, Riaan
South Africa, Stellenbosch
Stellenbosch University
Brevnova, Elena
United States, Lebanon
Mascoma Corporation
McBride, John E.
United States, Lebanon
Mascoma Corporation
Wiswall, Erin
United States, Lebanon
Mascoma Corporation
Froehlich, Allan
United States, Lebanon
Mascoma Corporation
Koivula, Anu
Finland, Espoo
Teknologian Tutkimuskeskus Vtt
Voutilainen, Sanni P.
Finland, Espoo
Teknologian Tutkimuskeskus Vtt
Siika-Aho, Matti
Finland, Espoo
Teknologian Tutkimuskeskus Vtt
La Grange, Daniel C.
South Africa, Stellenbosch
Stellenbosch University
Thorngren, Naomi
United States, Lebanon
Mascoma Corporation
Ahlgren, Simon
United States, Lebanon
Mascoma Corporation
Mellon, Mark
United States, Lebanon
Mascoma Corporation
Deleault, Kristen
United States, Lebanon
Mascoma Corporation
Rajgarhia, Vineet
United States, Lebanon
Mascoma Corporation
France, Paris
Total S.a.
van Zyl, W. H.
South Africa, Stellenbosch
Stellenbosch University
Penttilä, Merja
Finland, Espoo
Teknologian Tutkimuskeskus Vtt
Statistics
Citations: 164
Authors: 17
Affiliations: 4
Identifiers
Doi:
10.1186/1754-6834-4-30
ISSN:
17546834
Research Areas
Genetics And Genomics