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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Rapid diagnosis of acute hemorrhagic conjunctivitis due to coxsackievirus A24 variant by real-time one-step RT-PCR
Journal of Virological Methods, Volume 142, No. 1-2, Year 2007
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Description
Coxsackievirus A24 variant is, together with enterovirus 70 and adenoviruses, the major etiological agent involved in acute hemorrhagic conjunctivitis outbreaks worldwide. However, the standard virus isolation method followed by serotyping or VP1 region sequencing is time-consuming. A rapid method for the detection of coxsackievirus A24 variant from conjunctival swab specimens would be useful in the context of explosive and extensive outbreaks. A one-step real-time RT-PCR assay based on TaqMan technology was thus developed and assessed on 36 conjunctival swabs from outbreaks of conjunctivitis in Morocco in 2004 due to a coxsackievirus A24 variant and in Corsica in 2006 due to adenovirus type 3, and 83 virus strains including 41 coxsackievirus A24 variant collected in French Guiana and Guadeloupe in 2003, in the Democratic Republic of the Congo in 2003, in Morocco in 2004 and 42 other virus species genetically close or known to be responsible for conjunctivitis. All the conjunctival swabs from coxsackievirus A24 variant related outbreak and the 41 coxsackievirus A24 variant strains were tested positive by the RT-PCR assay within 4 h. This novel single-tube real-time RT-PCR assay is sensitive and specific, and consists in a reliable and faster alternative to the viral culture for recent and future acute hemorrhagic conjunctivitis outbreaks caused by coxsackievirus A24 variant. © 2007 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Lévêque, Nicolas
France, Lyon
Hopital Edouard Herriot
France, Villeurbanne
Université Claude Bernard Lyon 1
Lahlou-Amine, Idriss
Morocco, Agdal Rabat
Hôpital D'instruction Des Armées Mohamed V
Tcheng, Rémy
France, Lyon
Hopital Edouard Herriot
Falcon, Delphine
France, Lyon
Hopital Edouard Herriot
Rivat, Nathalie
France, Lyon
Hopital Edouard Herriot
France, Villeurbanne
Université Claude Bernard Lyon 1
Dussart, Philippe
French Guiana, Cayenne
Institut Pasteur de la Guyane
Muyembe-Tamfum, Jean Jacques
Congo
Institut National de Recherche Biomédicale
Chomel, Jean Jacques
France, Lyon
Hopital Edouard Herriot
France, Villeurbanne
Université Claude Bernard Lyon 1
Norder, Heléne
Sweden, Solna
Public Health Agency of Sweden
Eugene, Maxime
Belgium, Seraing
Eurogentec S.a.
Lina, Bruno
France, Lyon
Hopital Edouard Herriot
France, Villeurbanne
Université Claude Bernard Lyon 1
Statistics
Citations: 11
Authors: 11
Affiliations: 7
Identifiers
Doi:
10.1016/j.jviromet.2007.01.009
ISSN:
01660934
Study Locations
Congo
Morocco