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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
The heteroplasmic m.14709T>C mutation in the tRNA
Glu
gene in two Tunisian families with mitochondrial diabetes
Journal of Diabetes and its Complications, Volume 24, No. 4, Year 2010
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Description
Diabetes mellitus (DM) is a heterogeneous disorder characterized by the presence of chronic hyperglycemia. Genetic factors play an important role in the development of this disorder, and several studies reported mutations in nuclear genes implicated in the insulin function. Besides, DM can be maternally transmitted in some families, possibly due to the maternal mitochondrial inheritance. In fact, mitochondrial genes may be plausible causative agents for diabetes, since mitochondrial oxidative phosphorylation plays an important role in glucose-stimulated insulin secretion from beta cells. Materials and Methods: In this report, we screened two Tunisian families with mitochondrial diabetes for the m.3243A>G and the m.14709T>C mutations, respectively, in the tRNALeu(UUR) and the tRNAGlu genes. Results: The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and the sequence-specific primers by polymerase chain reaction (SSP-PCR) analysis in the leucocytes and the buccal mucosa in the members of the two families showed the absence of the m.3243A>G mutation and the presence of the heteroplasmic m.14709T>C mutation in the tRNAGlu gene in the two tested tissues. Conclusions: We conclude that the m.14709T>C mutation in the tRNAGlu gene could be a cause of mitochondrial diabetes in Tunisian affected families. In addition, the heteroplasmic loads correlated with the severity and the onset of mitochondrial diabetes in one family but not in the other, suggesting the presence of environmental factors or nuclear modifier genes. © 2010 Elsevier Inc. All rights reserved.
Authors & Co-Authors
Mezghani, Najla
Tunisia, Sfax
Faculty of Medicine of Sfax
Mkaouar-Rebai, Emna
Tunisia, Sfax
Faculty of Medicine of Sfax
Mnif, Mouna Feki
Tunisia, Sfax
Chu Habib Bourguiba
Charfî, Nadia
Tunisia, Sfax
Chu Habib Bourguiba
Rekik, Nabila Mejdoub
Tunisia, Sfax
Chu Habib Bourguiba
Youssef, Saoussan
Tunisia, Sfax
Faculty of Medicine of Sfax
Abid, Mohamed
Tunisia, Sfax
Chu Habib Bourguiba
Fakhfakh, Faiza
Tunisia, Sfax
Faculty of Medicine of Sfax
Statistics
Citations: 31
Authors: 8
Affiliations: 2
Identifiers
Doi:
10.1016/j.jdiacomp.2009.11.002
ISSN:
10568727
Research Areas
Cancer
Genetics And Genomics
Maternal And Child Health
Noncommunicable Diseases