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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Antisense oligonucleotide inhibition of hepatitis C virus genotype 4 replication in HepG2 cells
Cancer Cell International, Volume 6, Article 18, Year 2006
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Description
Background: Hepatitis C (HCV) viral infection is a serious medical problem in Egypt and it has a devastating impact on the Egyptian economy. It is estimated that over 15% of Egyptians are infected by the virus and thus finding a cure for this disease is of utmost importance. Current therapies for hepatitis C virus (HCV) genotype 4 with interferon/ ribavirin have not been successful and thus the development of alternative therapy for this genotype is disparately needed. Results: Although previous studies utilizing viral subgenomic or full cDNA fragments linked to reporter genes transfected into adhered cells or in a cell free system showed promise, demonstration of efficient viral replication was lacking. Thus, we utilized HepG2 cells infected with native HCV RNA genomes in a replication competent system and used antisense phosphorothioate Oligonucleotides (S-ODN) against stem loop IIId and the AUG translation start site of the viral polyprotein precursor to monitor viral replication. We were able to show complete arrest of intracellular replication of HCV-4 at 1 uM S-ODN, thus providing a proof of concept for the potential antiviral activity of S-ODN on native genomic replication of HCV genotype 4. Conclusion: We have successfully demonstrated that by using two S-ODNs [(S-ODN1 (nt 326-348) and S-ODN-2 (nt 264-282)], we were able to completely inhibit viral replication in culture, thus confirming earlier reports on subgenomic constructs and suggesting a potential therapeutic value in HCV type 4. © 2006 El Awady et al; licensee BioMed Central Ltd.
Authors & Co-Authors
El Awady, Mostafa K.
Egypt, Giza
National Research Centre
Bader El Din, Noha G.
Egypt, Giza
National Research Centre
El-Garf, Wael T.
Egypt, Giza
National Research Centre
Youssef, Samar Samir
Egypt, Giza
National Research Centre
Omran, Moataza Hassan Assan
Egypt, Giza
National Research Centre
El Abd, Yasmine S.
Egypt, Giza
National Research Centre
Goueli, Said A.
United States, Madison
University of Wisconsin-madison
Statistics
Citations: 97
Authors: 7
Affiliations: 2
Identifiers
Doi:
10.1186/1475-2867-6-18
ISSN:
14752867
e-ISSN:
14752867
Research Areas
Genetics And Genomics
Infectious Diseases
Study Locations
Egypt