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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Methodology and transport medium for collection of Helicobacter pylori on a string test in remote locations
Helicobacter, Volume 10, No. 6, Year 2005
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Description
Background. Helicobacter pylori can be isolated from patients using the string test but contaminating oral and nasopharyngeal microflora need to be suppressed by rapid plating out onto selective culture media. Recently, use of this diagnostic method was enhanced by using a novel transport medium to collect specimens from subjects in a remote Australian clinic over 1300 km from the laboratory. Methods. Retrieved string tests were transported to the laboratory in chilled polystyrene boxes in 5 ml screw-cap bottles with 3 ml of a brain heart infusion broth plus antibiotics. These were 20 μg/ml vancomycin, 10 μg/ml trimethoprim, 10 μg/ml cefsulodin, and 10 μg/ml amphotericin B. A comparison was made between subjects who gargled with a chlorhexidine mouthwash before swallowing the string test and those who did not. Results. Forty-five urea breath test-positive subjects were tested and H. pylori was isolated from 34 of them. Successful culture was achieved from string tests that were in transit for up to 29 hours and where the maximum temperature in the transport box was 14 °C. The additional use of a mouthwash had a marked effect on the isolation rate. H. pylori was cultured from 75% of subjects who gargled but only from 39% who did not. Conclusions. This methodology and transport medium can broaden the use of the string test to more remote geographic areas where endoscopy is not feasible so that H. pylori isolates may still be obtained for diagnostic and epidemiologic studies. The value of this promising methodology of collection and transport should be assessed in a controlled study. © 2005 Blackwell Publishing Ltd.
Authors & Co-Authors
Windsor, Helen M.
Australia, Perth
The University of Western Australia
Abioye-Kuteyit, Emmanuel A.
Australia
Puntukurnu Aboriginal Medical Service
Nigeria, Ife
Obafemi Awolowo University
Marshall, Barry James
Australia, Perth
The University of Western Australia
Australia, Perth
Sir Charles Gairdner Hospital
Statistics
Citations: 15
Authors: 3
Affiliations: 4
Identifiers
Doi:
10.1111/j.1523-5378.2005.00355.x
ISSN:
10834389
e-ISSN:
15235378
Research Areas
Health System And Policy