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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Generation of insulin-producing cells from human bone marrow-derived mesenchymal stem cells: Comparison of three differentiation protocols
BioMed Research International, Volume 2014, Article 832736, Year 2014
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Description
Introduction. Many protocols were utilized for directed differentiation of mesenchymal stem cells (MSCs) to form insulin-producing cells (IPCs). We compared the relative efficiency of three differentiation protocols. Methods. Human bone marrow-derived MSCs (HBM-MSCs) were obtained from three insulin-dependent type 2 diabetic patients. Differentiation into IPCs was carried out by three protocols: conophylline-based (one-step protocol), trichostatin-A-based (two-step protocol), and β-mercaptoethanol-based (three-step protocol). At the end of differentiation, cells were evaluated by immunolabeling for insulin production, expression of pancreatic endocrine genes, and release of insulin and c-peptide in response to increasing glucose concentrations. Results. By immunolabeling, the proportion of generated IPCs was modest (≃3%) in all the three protocols. All relevant pancreatic endocrine genes, insulin, glucagon, and somatostatin, were expressed. There was a stepwise increase in insulin and c-peptide release in response to glucose challenge, but the released amounts were low when compared with those of pancreatic islets. Conclusion. The yield of functional IPCs following directed differentiation of HBM-MSCs was modest and was comparable among the three tested protocols. Protocols for directed differentiation of MSCs need further optimization in order to be clinically meaningful. To this end, addition of an extracellular matrix and/or a suitable template should be attempted. © 2014 Mahmoud M. Gabr et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC4000976/bin/832736.f1.pdf
Authors & Co-Authors
Gabr, Mahmoud M.
Egypt, Mansoura
Mansoura University, Urology and Nephrology Center
Zakaria, Mahmoud M.
Egypt, Mansoura
Mansoura University, Urology and Nephrology Center
Refaie, Ayman Fathi
Egypt, Mansoura
Mansoura University, Urology and Nephrology Center
Khater, Sherry M.
Egypt, Mansoura
Mansoura University, Urology and Nephrology Center
Ashamallah, Sylvia A.
Egypt, Mansoura
Mansoura University, Urology and Nephrology Center
Ismail, A. M.
Egypt, Mansoura
Mansoura University, Urology and Nephrology Center
El-Badri, Nagwa S.
Egypt, 6th October
University of Science and Technology
Ghoneim, Mohamed A.
Egypt, Mansoura
Mansoura University, Urology and Nephrology Center
Statistics
Citations: 45
Authors: 8
Affiliations: 2
Identifiers
Doi:
10.1155/2014/832736
ISSN:
23146133
e-ISSN:
23146141
Research Areas
Cancer