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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Phase II evaluation of sensitivity and specificity of PCR and NASBA Followed by Oligochromatography for Diagnosis of Human African Trypanosomiasis in Clinical Samples from D.R. Congo and Uganda
PLoS Neglected Tropical Diseases, Volume 4, No. 7, Article e737, Year 2010
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Description
Background: The polymerase chain reaction (PCR) and nucleic acid sequence-based amplification (NASBA) have been recently modified by coupling to oligochromatography (OC) for easy and fast visualisation of products. In this study we evaluate the sensitivity and specificity of the PCR-OC and NASBA-OC for diagnosis of Trypanosoma brucei gambiense and Trypanosoma brucei rhodesiense human African trypanosomiasis (HAT). Methodology and Results: Both tests were evaluated in a case-control design on 143 HAT patients and 187 endemic controls from the Democratic Republic of Congo (DRC) and Uganda. The overall sensitivity of PCR-OC was 81.8% and the specificity was 96.8%. The PCR-OC showed a sensitivity and specificity of 82.4% and 99.2% on the specimens from DRC and 81.3% and 92.3% on those from Uganda. NASBA-OC yielded an overall sensitivity of 90.2%, and a specificity of 98.9%. The sensitivity and specificity of NASBA-OC on the specimens from DRC was 97.1% and 99.2%, respectively. On the specimens from Uganda we observed a sensitivity of 84.0% and a specificity of 98.5%. Conclusions/Significance: The tests showed good sensitivity and specificity for the T. b. gambiense HAT in DRC but rather a low sensitivity for T. b. rhodesiense HAT in Uganda. © 2010 Matovu et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC2897845/bin/pntd.0000737.s001.doc
Authors & Co-Authors
Matovu, Enock
Uganda, Kampala
Makerere University
Mugasa, Claire Mack
Uganda, Kampala
Makerere University
Netherlands, Amsterdam
Royal Tropical Institute - Kit
Ali Ekangu, Rosine
Democratic Republic Congo, Kinshasa
Institut National de Recherche Biomédicale Inrb
Deborggraeve, Stijn
Belgium, Antwerpen
Prins Leopold Instituut Voor Tropische Geneeskunde
Belgium, Leuven
Rega Institute for Medical Research
Lubega, George William
Uganda, Kampala
Makerere University
Laurent, Thierry Cl
Belgium, Gembloux
Coris Bioconcept
Schoone, Gerard J.
Netherlands, Amsterdam
Royal Tropical Institute - Kit
Schallig, H. D. F. H.
Netherlands, Amsterdam
Royal Tropical Institute - Kit
Büscher, Philippe
Belgium, Antwerpen
Prins Leopold Instituut Voor Tropische Geneeskunde
Statistics
Citations: 30
Authors: 9
Affiliations: 6
Identifiers
Doi:
10.1371/journal.pntd.0000737
Study Design
Case-Control Study
Study Locations
Congo
Uganda